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Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling
Published on: October 28, 2014
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ONE-seq: epitranscriptome and gene-specific profiling of NAD-capped RNA.
Kongyan Niu1,2, Jinyang Zhang1,2, Shuwen Ge1,2
1Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 100 Hai Ke Rd., Pudong, Shanghai 201210, China.
Nucleic Acids Research
|December 8, 2022
Summary
Nicotinamide adenine dinucleotide (NAD) can cap RNA, forming NAD-capped RNAs (NAD-RNAs). A new method, ONE-seq, identifies these NAD-RNAs, revealing their age-related changes in mouse liver.
Area of Science:
- Molecular Biology
- RNA Biology
- Aging Research
Background:
- Nicotinamide adenine dinucleotide (NAD) is a crucial metabolite involved in aging and disease.
- NAD can initiate RNA synthesis, forming NAD-capped RNAs (NAD-RNAs).
- The identity and function of NAD-RNAs in adult and aging tissues remain largely unknown.
Purpose of the Study:
- To develop a method for identifying NAD-capped RNAs (NAD-RNAs) in adult and aging tissues.
- To investigate the role of NAD-RNAs in aging and age-related diseases.
- To characterize the epitranscriptome-wide dynamics of NAD-RNAs.
Main Methods:
- Introduction of the ONE-seq method for NAD-RNA identification.
- ONE-seq utilizes a one-step chemo-enzymatic biotinylation, streptavidin capture, and NudC-catalyzed elution.
- Specific recovery of NAD-capped RNAs for epitranscriptome and gene-specific analyses.
Main Results:
- Discovery of over a thousand previously unknown NAD-RNAs in mouse liver.
- Revealed epitranscriptome-wide dynamics of NAD-RNAs associated with aging.
- Demonstrated that ONE-seq can identify NAD-RNAs responsive to physiological states.
Conclusions:
- The ONE-seq method enables specific identification and analysis of NAD-RNAs.
- Age-related changes in NAD-RNA profiles were uncovered in mouse liver.
- ONE-seq facilitates functional investigations into the role of NAD-RNAs in aging and disease.
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