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Updated: Aug 18, 2025
Automated Counterflow Centrifugal System for Small-Scale Cell Processing
Published on: December 12, 2019
Safe peripheral blood stem cell collection in patients less than 10 kg: A single-center review
Bryce E Pasko1,2, Melkon DomBourian1,2, Louise Helander2,3
1Department of Pathology, University of Colorado-Anschutz Medical Campus, Aurora, Colorado, USA.
Introduction:
Peripheral blood stem cell collection (PBSCC) is well-documented in adults and pediatric patients with larger total blood volume (TBV). However, very little data are available for the successful PBSCC of pediatric patients weighing less than 10 kg. Here, we highlight our institutional approach to PBSCC in this smaller-sized patient population.
Methods:
Our protocol, including blood prime, was reviewed for PBSCC for bone marrow transplantation (BMT) in 18 children weighing 4.5-9.9 kg who safely underwent 37 PBSCC procedures at a single institution, Children's Hospital Colorado, between September 2016 and February 2022.
Results:
We attained the individualized collection goals in all 18 patients with an average yield of 17.03 million CD34+ cells/kg of patient body weight (range: 0.84-67.45 million/kg). The average collection efficiency of the procedures was 41.5% (range: 23.0%-71.5%). We performed all 37 procedures safely and without complication. The estimated average TBV was 587 mL (range: 351-765 mL), the average blood volume processed was 596 mL (range: 351-756 mL), and the average TBVs processed was 2.5 (range: 1-4).
Conclusion:
PBSCC in patients ranging from 4.5 to 9.9 kg is safe and effective for collecting peripheral blood stem cells for BMT.
