Related Experiment Video

Updated: Aug 18, 2025

In Vitro Establishment of a Genetically Engineered Murine Head and Neck Cancer Cell Line using an Adeno-Associated Virus-Cas9 System
05:45

In Vitro Establishment of a Genetically Engineered Murine Head and Neck Cancer Cell Line using an Adeno-Associated Virus-Cas9 System

Published on: January 9, 2020

8.0K

Description of CRISPR-Cas9 development and its prospects in human papillomavirus-driven cancer treatment

Yuhao Wei1,2, Zhen Zhao1,2, Xuelei Ma1

  • 1Department of Biotherapy, Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

Frontiers in Immunology
|December 8, 2022
PubMed

Insights

Gene therapy using CRISPR-Cas9 offers a promising approach to combat human papillomavirus (HPV)-driven cancers by correcting genetic errors at the molecular level. This technology shows potential for efficient and precise cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Therapy

Background:

  • Human papillomaviruses (HPVs) are established causes of various cancers, termed HPV-driven cancers.
  • Gene therapy presents a strategy to address HPV-mediated carcinogenesis by correcting specific genetic defects.
  • CRISPR-Cas9 is an emerging gene-editing tool with the capacity for efficient and straightforward genomic modification.

Purpose of the Study:

  • To review existing research on CRISPR-Cas9-based gene therapies for HPV-driven cancers.
  • To explore the potential applications of CRISPR-Cas9 technology in the field of cancer gene therapy.

Main Methods:

  • Systematic overview of studies investigating CRISPR-Cas9 for HPV-driven cancer treatment.
  • Analysis of CRISPR-Cas9's capabilities in altering gene expression and cellular function.

Main Results:

  • CRISPR-Cas9 demonstrates efficiency in correcting genomic errors relevant to cancer development.
  • The technology allows for precise modification of gene expression and function in affected cells.

Conclusions:

  • CRISPR-mediated gene editing holds significant promise as a therapeutic strategy for HPV-driven cancers.
  • Further research into CRISPR-Cas9 applications could lead to novel gene therapy approaches for cancer treatment.
Keywords:
cancer treatmentclustered regularly interspaced short palindromic repeat/CRISPR-associated nuclease 9gene editinghuman papillomavirustumor microenvironment

More Related Videos

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells12:04

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

Published on: March 10, 2023

3.7K
In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

6.5K

Related Concept Videos

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.7K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
131
Cancer Vaccines01:30

Cancer Vaccines

Cancer treatment vaccines are a rapidly evolving field that offers a promising approach to immunotherapy. Unlike traditional vaccines that prevent diseases, cancer treatment vaccines are designed to treat existing cancers by stimulating the immune system to recognize and attack cancer cells.
Cancer vaccines come in two categories: preventive (prophylactic) and treatment (active). Preventive vaccines, such as the Human Papillomavirus (HPV) vaccine, protect against viruses that cause certain...
480
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.3K
Mechanisms of Retrovirus-induced Cancers01:51

Mechanisms of Retrovirus-induced Cancers

Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
5.2K
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.8K