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Related Experiment Video

Updated: Aug 18, 2025

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
11:02

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

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An interoperability framework for multicentric breath metabolomic studies.

Amanda Gisler1, Kapil Dev Singh1,2, Jiafa Zeng1,2,3

  • 1University Children's Hospital Basel UKBB, University of Basel, 4056 Basel, Switzerland.

Iscience
|December 8, 2022
PubMed
Summary
This summary is machine-generated.

This study presents an interoperability framework for breath analysis, standardizing multicenter data acquisition. It reveals technical variability of ~20% and identifies ~850 core human exhaled metabolome features, paving the way for clinical trials.

Keywords:
Health sciencesclinical findingfinding by sitehealth information managementmedical information practitioner

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Metabolomics

Background:

  • Exhaled breath analysis holds potential for precision medicine.
  • Limited validation in multicenter trials hinders clinical translation of breath tests.

Purpose of the Study:

  • To develop and apply an interoperability framework for standardized multicenter breath analysis.
  • To assess technical variability and metabolic coverage in exhaled breath.

Main Methods:

  • Utilized secondary electrospray ionization-high resolution mass spectrometry (SEI-HRMS).
  • Implemented a standardized interoperability framework for multicenter data acquisition and processing.

Main Results:

  • Technical variability across multicenter data was approximately 20%.
  • Identified a core human exhaled metabolome signature of ~850 features, primarily in amino acid, xenobiotic, and carbohydrate pathways.
  • Observed high inter-subject variability in certain metabolic classes (e.g., amino acids, fatty acids) but stability in others (e.g., TCA cycle).

Conclusions:

  • The developed interoperability framework enables standardized multicenter breath analysis.
  • The findings provide an overview of metabolic coverage in exhaled breath.
  • This work facilitates future large-scale multicenter trials for clinical validation of breath tests.