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Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
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Tuberculosis detection from raw sputum samples using Au-electroplated screen-printed electrodes as E-DNA sensor.

M N Sharif1,2, S Taufiq1,2, M Sohail3

  • 1Biosensors and Therapeutics Lab, School of Interdisciplinary Engineering and Sciences (SINES), Islamabad, Pakistan.

Frontiers in Chemistry
|December 8, 2022
PubMed
Summary
This summary is machine-generated.

This study developed a novel electrochemical DNA biosensor for detecting Mycobacterium tuberculosis. The ultrasensitive device uses gold nanoparticles on screen-printed electrodes for improved diagnostics, aiding TB eradication efforts.

Keywords:
IS-6110electrochemical biosensingmtb detectionscreen printed electrode (SPE)tuberculosis

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Area of Science:

  • Biosensor Technology
  • Nanomaterials in Diagnostics
  • Molecular Detection

Background:

  • Tuberculosis (TB) is a major global health threat, particularly in developing countries.
  • Effective diagnosis is hindered by the lack of sensitive and rapid detection tools.
  • Current diagnostic methods often require complex procedures and specialized equipment.

Purpose of the Study:

  • To develop a label-free, ultrasensitive electrochemical DNA biosensor for Mycobacterium tuberculosis detection.
  • To enhance signal amplification and probe immobilization using gold nanoparticles on screen-printed electrodes.
  • To create a potential platform for disposable biosensors for direct detection from clinical samples.

Main Methods:

  • Electrochemical deposition of gold nanoparticles onto screen-printed electrodes.
  • Immobilization of single-stranded DNA (ssDNA) probes via Au-S bonds.
  • Characterization using SEM/EDX and XRD; electrochemical analysis with CV and DPV.

Main Results:

  • The modified electrodes showed enhanced conductivity and facilitated DNA probe immobilization.
  • Differential pulse voltammetry (DPV) demonstrated a strong correlation (R² = 0.97) with target DNA concentrations.
  • The biosensor achieved a detection range of 2-10 nM with a low limit of detection (1.91 nM) and high selectivity.

Conclusions:

  • The developed electrochemical DNA biosensor offers a sensitive and selective method for Mycobacterium tuberculosis detection.
  • The use of gold nanoparticles and screen-printed electrodes provides signal amplification and facilitates probe immobilization.
  • This technology has the potential for developing disposable biosensors for rapid TB diagnosis directly from sputum samples, bypassing DNA purification.