Related Experiment Video

Updated: Aug 18, 2025

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells
09:47

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

Published on: December 9, 2022

3.6K

Generation of Human iPSC-Derived Retinal Organoids for Assessment of AAV-Mediated Gene Delivery

Amy Tso1, Bruna Lopes da Costa1,2,3, Alexandra Fehnel1,2,3

  • 1Edward S. Harkness Eye Institute, Department of Ophthalmology, Columbia University Irving Medical Center/New York-Presbyterian Hospital, New York, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 9, 2022
PubMed

Keywords:
3D retinal organoidsAMASS (agarose microwell array seeding and scraping)Adeno-associated virus (AAV)Cell tropismGene therapyInduced pluripotent stem cells (iPSCs)Retinal pigment epithelium (RPE)Transgene expression assay

More Related Videos

Directed Induction of Retinal Organoids from Human Pluripotent Stem Cells06:38

Directed Induction of Retinal Organoids from Human Pluripotent Stem Cells

Published on: April 21, 2021

3.0K
Defined Xeno-free and Feeder-free Culture Conditions for the Generation of Human iPSC-derived Retinal Cell Models11:29

Defined Xeno-free and Feeder-free Culture Conditions for the Generation of Human iPSC-derived Retinal Cell Models

Published on: September 6, 2018

9.5K

Related Concept Videos

iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
2.8K