Three-dimensional evaluation of microvascular networks using contrast-enhanced ultrasound and microbubble tracking

Evaluating tumor microvascular networks with use of contrast-enhanced ultrasound (CEUS) imaging and one-dimensional (1D) linear array transducers have inherit limitations as tumors exist in volume space. The use of a mechanical sweep allows users to overcome this limitation. To that end, we have developed a new method by which a 1D linear array transducer can be mechanically scanned over a region-of-interest to capture a volume of data allowing for the evaluation of microvasculature structures in 3D space. After intravascular injection of a microbubble (MB) contrast agent into a developing chicken embryo, a sequence of CEUS images were acquired using a Vevo 3100 scanner (VisualSonics Inc) and taken at multiple tissue cross-sections. The CEUS images were processed with a singular value filter (SVF) to help remove any clutter signal. MB localization was performed, and frame-to-frame MB movement was analyzed to produce spatial maps depicting blood flow and velocity at each tissue cross-section. Reconstruction of all images allowed visualization of microvascular networks and blood velocity distribution in volume space.