Blood serum analysis: A modified sandwich enzyme-linked immunosorbent assay protocol

Area of Science:

• Biochemistry
• Immunology
• Biotechnology

Background:

• Blood serum analysis is crucial for diagnostics, in vivo research, and clinical studies.
• Enzyme-linked immunosorbent assay (ELISA) is a standard method for quantifying serum cytokine levels.
• Commercial ELISA kits are expensive and often unavailable for novel or uncommon targets.

Purpose of the Study:

• To present a modified ELISA protocol for versatile blood serum analysis.
• To offer a cost-effective alternative to commercial kits.
• To enable the measurement of novel or unique serum targets.

Main Methods:

• Development and standardization of a modified ELISA protocol.
• Application of the protocol for measuring various blood serum targets.
• Validation of the method for reliability and cost-effectiveness.

Main Results:

• The modified ELISA protocol can measure blood serum levels of any target once standardized.
• This method significantly minimizes the expense associated with commercial ELISA kits.
• The protocol is effective for novel or unique targets lacking commercial options.

Conclusions:

• The modified ELISA method provides an efficient and cost-effective approach for serum cytokine measurements.
• This technique supplements clinical and in vivo studies with reliable data.
• The protocol offers a valuable tool for researchers studying diverse serum targets.