[Primary structure of tRNAAsn of bacteriophage T5]

Unformly 32P-labelled phage-specific tRNAAsn was isolated from bacteriophage T5-infected E. coli cells its oligonucleotide fragments were fractionated by thin-layer chromatography on cellulose and the tRNA's primary structure was determined as follows: (formula; see text). Main features of the structure are: displacement of the constant residue A14 by U; absence of G27 X A43 and U30 X psi40 pairing in the anticodon stem; possibility of additional pairing in D-loop. Comparison of T5 tRNAAsn with other asparagine tRNAs is presented.