[Primary structure of proline tRNA of bacteriophage T5]

The uniformly 32P-labeled bacteriophage T5 proline tRNA has been isolated from phage-infected E. coli cells by two-dimensional PAGE. Its nucleotide sequence has been determined by conventional techniques (using TLC on cellulose for oligonucleotide fractionation) as follows: (Formula: see text). The tRNA has the anticodon sequence UGG, which can presumably recognize the four proline-specific codons (CCN). It has 70% homology with phage T4 tRNA(Pro).