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"Runde" virus: further characteristics and a method for purification.
Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology
|December 1, 1978
Summary
Quantifying Runde virus proved challenging in cell cultures. Newborn mouse brains yielded higher virus titers, with optimal growth observed in BHK 21/c13 cells.
Area of Science:
- Virology
- Cell Biology
Background:
- Quantifying Runde virus in cell cultures presents challenges due to low and irreproducible titers.
- Newborn mouse brains offer a more effective source for higher viral yields compared to cell cultures.
Purpose of the Study:
- To investigate optimal methods for Runde virus quantification and characterization.
- To analyze the growth kinetics and properties of Runde virus in cell culture and animal models.
Main Methods:
- Comparative titration of Runde virus in BHK 21/c13, BSC-1, Vero, and GMK cells versus newborn mouse brains.
- Growth curve analysis in BHK 21/c13 cells, monitoring viral titers over time.
- Assessment of viral genome type through resistance to BUdR.
- Virus concentration and purification using polyethylene glycol 6000/NaCl precipitation and hydroxylapatite chromatography.
- Hemadsorption assays and characterization of hemagglutinins.
- Treatment of virus preparations with colloidal silica gel to broaden erythrocyte agglutination spectrum.
Main Results:
- BHK 21/c13, BSC-1, Vero, and GMK cells yielded low and inconsistent Runde virus titers.
- Newborn mouse brains provided significantly higher virus yields.
- In BHK 21/c13 cells, viral titers reached a maximum of approximately 10(6) baby mouse LD50 at 48-72 hours post-infection, followed by a gradual decline.
- Runde virus demonstrated resistance to BUdR, indicating an RNA genome.
- Polyethylene glycol precipitation and hydroxylapatite chromatography enabled virus concentration and purification.
- Colloidal silica gel treatment expanded the range of agglutinable erythrocytes.
Conclusions:
- Newborn mouse brains are superior to the tested cell lines for Runde virus quantification.
- BHK 21/c13 cells support Runde virus replication, but with limitations in achieving high titers and sustained infectivity.
- The virus possesses an RNA genome and can be effectively concentrated and purified using established biochemical methods.