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Manganese and the cGAS-STING Pathway in Infectious Mononucleosis Caused by Epstein-Barr Virus Infection

Jinjin Shi1,2, Yuqin Li1, Dandan Zhang1

  • 1Department of Infectious Disease, Children's Hospital of Soochow University, Soochow University, Suzhou, China.

Viral Immunology
|December 9, 2022
PubMed

Insights

Manganese (Mn) levels are elevated in children with infectious mononucleosis (IM) and correlate with the cGAS-STING pathway, suggesting a role in Epstein-Barr virus (EBV) immune pathogenesis.

Area of Science:

  • Immunology
  • Virology
  • Pediatrics
  • Environmental Health

Background:

  • Infectious mononucleosis (IM) is a common viral illness caused by the Epstein-Barr virus (EBV).
  • The innate immune response, involving the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway, is crucial in viral infections.
  • Manganese (Mn) is an essential trace element with potential immunomodulatory roles.

Purpose of the Study:

  • To investigate the clinical significance of serum manganese (Mn) levels in children with EBV-induced infectious mononucleosis (IM).
  • To analyze the correlation between Mn levels and the cGAS-STING pathway in IM.
  • To explore the immune pathogenesis of EBV infection involving Mn and the cGAS-STING pathway.

Main Methods:

  • Serum Mn levels were quantified using inductively coupled plasma mass spectrometry.
  • mRNA expression of cGAS, STING, TBK1, IRF3, and inflammatory factors was measured by real-time RT-PCR.
  • Serum Interferon-alpha (IFN-α) and IFN-beta (IFN-β) levels were determined by ELISA.

Main Results:

  • Children with IM exhibited significantly higher serum Mn levels compared to healthy controls.
  • Elevated expression of cGAS, STING, and inflammatory factors was observed in children with IM.
  • Serum Mn levels positively correlated with cGAS expression and inflammatory factor levels in IM patients.

Conclusions:

  • Serum manganese may play a significant role in the immune response during EBV-induced infectious mononucleosis in children.
  • The cGAS-STING pathway and associated inflammatory cytokines are implicated in the immune pathogenesis of IM.
  • Mn, cGAS, STING, and inflammatory cytokines are potential contributors to the immune mechanisms underlying IM.
Keywords:
Epstein-Barr virusMncGAS–STING signaling pathwayimmune responseinfectious mononucleosis

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