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Updated: Aug 18, 2025

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
Published on: January 20, 2022
A cyclodextrin-based reagent for cis/trans-geometrical isomers separation by mobility measurements and chemical
Fangling Wu1, Xishi Wu2, Zhe Li1
1Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China.
Abstract:
Identification of cis/trans-carbon-carbon double-bond (CC) isomers remain challenging. Herein, a simple and rapid method for the separation and analysis of cis/trans-maleic acid (MA) and aconitic acid (AA) using Trapped Ion Mobility Spectrometry (TIMS) was developed. α-, β-, γ-cyclodextrin (CD) were served as the separation reagent, slight difference in mobility separation was obtained by [CD-MA/AA-H]-. Specially, with the addition of divalent metal ion (G2+) as coordination metal ion, the separation effect was much increased by [CD-MA/AA + G-H]+, and α-CD has better mobility separation effect than β-/γ-CD. Moreover, chemical calculations revealed the binary and ternary complexes are in the inclusion forms, and microscopic interactions between cis/trans-MA/AA, CDs, and G2+ are somewhat different that making their mobility separation. Finally, quantifications of cis/trans-isomers were analyzed in food samples, with good linearity (R2 > 0.99) and recoveries obtained from 87.25 % to 100.73 %.
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