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High-throughput Detection Method for Influenza Virus
Published on: February 4, 2012
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DIAGNOSTIC CAPACITY OF DETECTION OF SPECIFIC ANTIBODIES TO PANDEMIC INFLUENZA A(H1N1)PDM09 VIRUS
E A Mukasheva1, L I Nikolaeva1, P I Makhnovsky1
1D.I. Ivanovsky Institute of Virology, Federal State Budgetary Institution «Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya».
Voprosy Virusologii
|December 10, 2022
Summary
New ELISA test systems accurately detect influenza A(H1N1)pdm09 antibodies. These systems are more sensitive than traditional methods, enabling better monitoring of IgG antibody dynamics during infection.
Area of Science:
- Virology
- Immunology
- Diagnostic Medicine
Background:
- Serological studies are crucial for diagnosing influenza.
- Influenza A(H1N1)pdm09 requires accurate diagnostic tools.
- Traditional methods like Hemagglutination Inhibition (HAI) have limitations.
Purpose of the Study:
- To analyze experimental ELISA test systems for detecting specific antibodies to influenza A(H1N1)pdm09.
- To compare the dynamics of these antibodies with the HAI method.
- To evaluate the accuracy, specificity, and sensitivity of the developed ELISA systems.
Main Methods:
- Utilized 20 paired serum samples from hospitalized patients with influenza A(H1N1)pdm09.
- Employed two ELISA test systems: one for IgM and one for IgG detection.
- Compared ELISA results with the traditional HAI method and a control group (hepatitis C patients).
Main Results:
- Developed ELISA systems demonstrated accuracy and specificity for detecting both IgM and IgG antibodies.
- Reliable dynamics of specific IgG titers were observed in 15 out of 20 paired sera, showing more pronounced increases than HAI.
- IgM antibodies were detectable up to 10 days, though dynamics were less reliable in paired samples.
Conclusions:
- The experimental ELISA test systems are accurate, specific, and more sensitive than HAI for detecting influenza A(H1N1)pdm09 antibodies.
- These ELISA systems allow for monitoring IgG dynamics, even when HAI shows no significant titer increase.
- The developed ELISA offers a superior tool for influenza A(H1N1)pdm09 diagnosis and monitoring.
