Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First-order field autocorrelation function analysis-based dynamic OCT for tissue viability assessment.

Biomedical optics express·2026
Same author

Cortical activity during narrative discourse production in individuals with post-stroke aphasia and controls measured via functional near-infrared spectroscopy.

medRxiv : the preprint server for health sciences·2026
Same author

Visual gamma stimulation causes prolonged enhancement of low-frequency blood flow oscillations across cortical regions in mice.

bioRxiv : the preprint server for biology·2026
Same author

Comparative validation of speckle contrast optical spectroscopy against diffuse correlation spectroscopy for monitoring human cerebral blood flow.

Neurophotonics·2026
Same author

Mapping Slow Speckle Dynamics to Probe Cellular Metabolic Activity In Vivo using Laser Speckle Contrast Imaging.

bioRxiv : the preprint server for biology·2026
Same author

Ostwald Ripening of Liquid-Metal-Grown Micropattern-Confined Crystals by Solid-Phase Diffusion.

Nano letters·2026

Related Experiment Video

Updated: Aug 18, 2025

Spatial Temporal Analysis of Fieldwise Flow in Microvasculature
09:39

Spatial Temporal Analysis of Fieldwise Flow in Microvasculature

Published on: November 18, 2019

5.9K

Measuring capillary flow dynamics using interlaced two-photon volumetric scanning.

John T Giblin1, Seong-Wook Park1, John Jiang1

  • 1Neurophotonics Center, Department of Biomedical Engineering, Boston University, Boston, MA, USA.

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
|December 10, 2022
PubMed
Summary

This study introduces a novel two-photon microscopy technique using Bessel beams and a unique scanning pattern to precisely measure red blood cell (RBC) flow speeds in the complex cerebral microvasculature.

Keywords:
Blood flowcapillaryflow heterogeneitystalltwo-photon microscopy

More Related Videos

In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette
10:54

In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette

Published on: June 7, 2019

7.6K
Meso-Scale Particle Image Velocimetry Studies of Neurovascular Flows In Vitro
08:00

Meso-Scale Particle Image Velocimetry Studies of Neurovascular Flows In Vitro

Published on: December 3, 2018

8.5K

Related Experiment Videos

Last Updated: Aug 18, 2025

Spatial Temporal Analysis of Fieldwise Flow in Microvasculature
09:39

Spatial Temporal Analysis of Fieldwise Flow in Microvasculature

Published on: November 18, 2019

5.9K
In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette
10:54

In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette

Published on: June 7, 2019

7.6K
Meso-Scale Particle Image Velocimetry Studies of Neurovascular Flows In Vitro
08:00

Meso-Scale Particle Image Velocimetry Studies of Neurovascular Flows In Vitro

Published on: December 3, 2018

8.5K

Area of Science:

  • Neuroscience
  • Biomedical Engineering
  • Optical Imaging

Background:

  • Traditional two-photon microscopy and OCT have limitations in imaging depth and specificity for cerebral microvasculature.
  • Raster scanning in these techniques restricts simultaneous measurement of red blood cell (RBC) velocities across multiple capillaries.
  • Studying RBC flow dynamics in the microvascular network is crucial for understanding various physiological and pathological conditions.

Purpose of the Study:

  • To develop an advanced imaging method for high-resolution, volumetric analysis of RBC flow in the cerebral microvasculature.
  • To overcome the depth-of-field and specificity limitations of existing imaging modalities.
  • To enable simultaneous, high-speed tracking of RBC velocities in multiple capillaries.

Main Methods:

  • Utilized a custom two-photon system employing an axicon-based Bessel beam for enhanced penetration and fluorescent specificity.
  • Implemented a novel scanning pattern to acquire image pairs with a short time delay for accurate RBC flow tracking.
  • Performed simultaneous tracking of RBC flow speeds in 10+ capillaries within a 237x237x120 µm³ volume at 1 Hz.

Main Results:

  • Quantified the spatial and temporal variability of RBC flow speeds in the microvasculature.
  • Successfully tracked RBC flow dynamics around capillary flow stalls.
  • Demonstrated the capability to measure flow speeds up to 300 µm in depth.

Conclusions:

  • The developed Bessel beam two-photon microscopy with a novel scan pattern significantly advances the study of microvascular RBC flow.
  • This technique provides unprecedented detail on RBC velocity dynamics, including spatial-temporal variations and responses to flow stalls.
  • Offers a powerful tool for investigating microvascular function and disease in animal models with improved accuracy and depth.