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Related Experiment Video

Updated: Aug 18, 2025

Microfluidics in Assessing Platelet Function
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Platelets from 13-lined ground squirrels are resistant to cold storage lesions.

N Splinter1, A Mancosky1, C Laffin1

  • 1Biology Department, University of Wisconsin-La Crosse, 1725 State St., La Crosse, WI, 54601, USA.

Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
|December 10, 2022
PubMed
Summary

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Ground squirrel platelets resist cold storage damage, unlike human platelets. This suggests hibernating animals have natural mechanisms for preserving platelet function during torpor and arousal.

Area of Science:

  • Comparative physiology
  • Hematology
  • Mammalian hibernation

Background:

  • Hibernation involves decreased heart rate and blood flow, increasing clot risk.
  • Mammalian platelets face cold storage lesions and rapid clearance, limiting human platelet transfusion shelf life to 7 days.
  • Ground squirrels sequester platelets in the liver during torpor, releasing them upon arousal.

Purpose of the Study:

  • To investigate the resistance of ground squirrel platelets to cold storage lesions compared to human platelets.
  • To elucidate potential mechanisms underlying platelet cold adaptation in hibernating mammals.

Main Methods:

  • Human and ground squirrel platelets were stored at room temperature or 4°C.
  • Analysis included phosphatidylserine exposure, caspase activation, and sialic acid residue cleavage.
Keywords:
Cold storage of plateletsHibernationPlatelet preservationTorporTransfusion

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  • Phagocytosis assays using HepG2 cells were performed post-storage.

    • Main Results:

    • Human platelets stored at 4°C showed increased phosphatidylserine exposure, caspase activation, and desialylation.
    • Cold-stored human platelets were readily phagocytosed by HepG2 cells.
    • Ground squirrel platelets exhibited minimal cold storage lesions, with low phagocytosis rates even after cold exposure.

    Conclusions:

    • Ground squirrel platelets demonstrate remarkable resistance to cold storage lesions compared to human platelets.
    • This resistance suggests a natural adaptation in hibernating species for maintaining platelet function during torpor.
    • Findings may inform strategies for improving human platelet storage and transfusion viability.