Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

10.3K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

OBERON3 and SUPPRESSOR OF MAX2 1-LIKE proteins form a regulatory module driving phloem development.

Nature communications·2023
Same author

COP1 dynamics integrate conflicting seasonal light and thermal cues in the control of <i>Arabidopsis</i> elongation.

Science advances·2022
Same author

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation.

Journal of visualized experiments : JoVE·2019
Same author

The Phloem as a Mediator of Plant Growth Plasticity.

Current biology : CB·2019
Same author

Bifacial cambium stem cells generate xylem and phloem during radial plant growth.

Development (Cambridge, England)·2019
Same author

A Comprehensive Toolkit for Inducible, Cell Type-Specific Gene Expression in Arabidopsis.

Plant physiology·2018

Related Experiment Video

Updated: Aug 18, 2025

Author Spotlight: Vascular Tissue Dissociation and Exploring Single-Cell Subclusters for Targeted Therapy
04:21

Author Spotlight: Vascular Tissue Dissociation and Exploring Single-Cell Subclusters for Targeted Therapy

Published on: January 19, 2024

2.9K

BD Rhapsody™ Single-Cell Analysis System Workflow: From Sample to Multimodal Single-Cell Sequencing Data.

Jannes Ulbrich1, Vadir Lopez-Salmeron2, Ian Gerrard2

  • 1BD Biosciences GmbH, Heidelberg, Germany. jannes.ulbrich@bd.com.

Methods in Molecular Biology (Clifton, N.J.)
|December 10, 2022
PubMed
Summary

Single-cell sequencing advances understanding of biological systems. The BD Rhapsody™ system offers multiomics analysis, measuring mRNA, protein, and immune repertoire from thousands of cells for comprehensive insights.

Keywords:
BD BiosciencesBD Rhapsody™ Single-Cell Analysis SystemBD® AbSeqMicrowellsMultimodal Single-Cell SequencingSingle-Cell Multiomics

More Related Videos

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.6K
Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
12:44

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Published on: November 11, 2014

12.4K

Related Experiment Videos

Last Updated: Aug 18, 2025

Author Spotlight: Vascular Tissue Dissociation and Exploring Single-Cell Subclusters for Targeted Therapy
04:21

Author Spotlight: Vascular Tissue Dissociation and Exploring Single-Cell Subclusters for Targeted Therapy

Published on: January 19, 2024

2.9K
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.6K
Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
12:44

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Published on: November 11, 2014

12.4K

Area of Science:

  • Life Science Research
  • Immunology
  • Genomics

Background:

  • Single-cell sequencing significantly advances biological system comprehension, including the immune system.
  • It overcomes limitations in fields like oncology, developmental biology, and neurobiology.
  • Multimodal analysis provides deeper insights than traditional methods.

Purpose of the Study:

  • To describe the complete BD Rhapsody™ Single-Cell Analysis System.
  • To highlight its capabilities in capturing multimodal information from single cells.
  • To provide guidance on sample preparation and data analysis.

Main Methods:

  • Utilizes microwell-based cartridges for single-cell capture.
  • Employs an imaging device for quality control (viability, multiplets).
  • Enables parallel measurement of mRNA, protein, T-cell receptors (TCR), and B-cell receptors (BCR) via next-generation sequencing (NGS).

Main Results:

  • The BD Rhapsody™ system enables multiomics analysis of thousands of single cells.
  • It integrates mRNA and protein expression, immune repertoire, and antigen-specific cell identification.
  • Simultaneous measurement provides a comprehensive single readout.

Conclusions:

  • The BD Rhapsody™ Single-Cell Analysis System offers a powerful platform for multiomics research.
  • It facilitates detailed analysis of cellular heterogeneity and function.
  • Researchers can access updated protocols and technical information online.