Highly sensitive and selective serotonin (5-HT) electrochemical sensor based on ultrafine Fe₃O₄ nanoparticles anchored on carbon spheres

Neurotransmitter serotonin (5-HT) is involved in various physiological and pathological processes. Therefore, its highly sensitive and selective detection in human serum is of great significance for early diagnosis of disease. In this work, employing iron phthalocyanine as Fe source, ultrafine Fe₃O₄ nanoparticles anchored on carbon spheres (Fe₃O₄/CSs) have been prepared, which exhibits an excellent electrochemical sensing performance toward 5-HT. With carbonecous spheres turned into conductive carbon spheres under the heat treatment in N₂ atmosphere, iron phthalocyanine absorbed on their surfaces are simultaneously pyrolysised and oxidized, and finally transformed into ultrafine Fe₃O₄ nanoparticles. Electrochemical results demonstrate a high sensitivity (5.503 μA μM^-1) and a low detection limit (4 nM) toward 5-HT for as-prepared Fe₃O₄/CSs. In combination with the morphology and physicochemical property of Fe₃O₄/CSs, the enhanced sensing mechanism toward 5-HT is disscussed. In addition, the developed electrochemical sensor also displays a good sensing stability and an anti-interferent ability. Further applied in real human serum samples, a satisfactory recovery rate is achieved. Promisingly, the developed electrochemical sensor can be employed for the determination of 5-HT in actual samples.