Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

5.8K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
5.8K
Biological Causes of Schizophrenia01:29

Biological Causes of Schizophrenia

115
Schizophrenia, a severe psychiatric disorder, arises from a complex interplay of biological factors, including genetic predisposition, structural brain abnormalities, neurotransmitter dysregulation, and developmental irregularities. These factors collectively contribute to the onset and progression of the disorder, which typically manifests in late adolescence or early adulthood.
Genetic Factors in Schizophrenia
The genetic basis of schizophrenia is strongly supported by family and twin...
115
Role of Septins01:02

Role of Septins

1.8K
Septins are the recently discovered fourth major protein component of the cytoskeleton, along with microfilaments, microtubules, and intermediate filaments. These proteins can associate with other cytoskeletal filaments and carry out varied roles or can be free-floating in the cytoplasm.
Cellular Functions of Septins
Recent studies have revealed the multifaceted roles of septins in various cellular processes such as cytokinesis, ciliogenesis, and neurogenesis. Septins act as scaffolds and...
1.8K
Cytoskeletal Linker Proteins - Plakins01:09

Cytoskeletal Linker Proteins - Plakins

2.4K
Plakins are large proteins with binding domains for microtubules, microfilaments, intermediate filaments, and membrane-associated protein complexes at cell junctions. Plakin functions are evolutionarily conserved and are primarily involved in organizing the different components of the cytoskeleton by crosslinking them to each other and connecting them to the cell-matrix and cell adhesion complexes. They are also known to interact with signal transducers, serve as scaffolds for signaling...
2.4K
Psychosis: Pathophysiology of Schizophrenia and Other Psychotic Disorders01:27

Psychosis: Pathophysiology of Schizophrenia and Other Psychotic Disorders

845
Schizophrenia is a neurodevelopmental disorder whose origins are rooted in complex genetic components. Despite our burgeoning understanding, the pathophysiology of this disorder remains incompletely deciphered.
Researchers have identified genetic factors that increase susceptibility to schizophrenia, underscoring the intricate interplay between genetics and environment in disease development. At the core of schizophrenia's pathophysiology is excessive dopaminergic neurotransmission within...
845
Postsynaptic Potential (PSP)01:32

Postsynaptic Potential (PSP)

3.1K
Postsynaptic potential (PSP) refers to a change in the electrical potential of a neuron when neurotransmitters released by presynaptic neurons bind to postsynaptic receptors. This potential can either be excitatory, leading to depolarization and ultimately action potential generation, or inhibitory, leading to hyperpolarization and suppression of the postsynaptic neuron.
There are two types of receptors: ionotropic and metabotropic.
The ionotropic receptor is the membrane protein that has an...
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Citrulline drives age-related lipid deposition for healthspan.

Nature communications·2026
Same author

Glucokinase Regulatory Protein Gene Polymorphism as a Predictive Biomarker for Early Renal Injury in Newly Diagnosed Early-Onset Type 2 Diabetes Mellitus.

Diabetes/metabolism research and reviews·2026
Same author

Insight into pseudocapacitive and electrical double-layer capacitive behaviors in polyaniline-based supercapacitors.

Journal of colloid and interface science·2026
Same author

The Natural Compound Cornuside Protects Against Acute Liver Failure Induced by Lipopolysaccharide and D-Galactosamine.

Drug design, development and therapy·2026
Same author

Enhanced Performance of an Au/MoS<sub>2</sub>/GaAs Photodetector by Room-Temperature Metal Electrode Transfer.

Nanomaterials (Basel, Switzerland)·2026
Same author

Relationship between the triglyceride-glucose index and non-alcoholic fatty liver disease among individuals with atrial fibrillation.

American journal of cardiovascular disease·2026

Related Experiment Video

Updated: Aug 18, 2025

Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient
08:06

Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient

Published on: September 3, 2014

31.4K

SAPAP Scaffold Proteins: From Synaptic Function to Neuropsychiatric Disorders.

Yunxia Bai1,2, Huimin Wang1,2,3, Chunxia Li1,2

  • 1Key Laboratory of Brain Functional Genomics (STCSM & MOE), Affiliated Mental Health Center (ECNU), School of Psychology and Cognitive Science, East China Normal University, Shanghai 200062, China.

Cells
|December 11, 2022
PubMed
Summary

Postsynaptic scaffolding proteins (SAPAP1-4) are crucial for brain function. Genetic mutations and dysfunction in these proteins are linked to various neurological and psychiatric disorders, impacting synaptic health.

Keywords:
SAPAP/DLGAP/GKAPanimal modelcognitive dysfunctionexcitatory synapseneuropsychiatric disorderspostsynaptic scaffolding protein

More Related Videos

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices
07:44

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices

Published on: October 6, 2017

17.3K
Presynapse Formation Assay Using Presynapse Organizer Beads and &ldquo;Neuron Ball&rdquo; Culture
10:17

Presynapse Formation Assay Using Presynapse Organizer Beads and “Neuron Ball” Culture

Published on: August 2, 2019

8.3K

Related Experiment Videos

Last Updated: Aug 18, 2025

Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient
08:06

Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient

Published on: September 3, 2014

31.4K
Evaluation of Synapse Density in Hippocampal Rodent Brain Slices
07:44

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices

Published on: October 6, 2017

17.3K
Presynapse Formation Assay Using Presynapse Organizer Beads and &ldquo;Neuron Ball&rdquo; Culture
10:17

Presynapse Formation Assay Using Presynapse Organizer Beads and “Neuron Ball” Culture

Published on: August 2, 2019

8.3K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Excitatory synaptic transmission is fundamental to brain activity and behavior.
  • Postsynaptic scaffolding proteins, SAPAP1-4, are key components of the postsynaptic density (PSD) at excitatory synapses.
  • SAPAPs are vital for synaptic structure, development, plasticity, and signaling.

Purpose of the Study:

  • To review recent genetic, epigenetic, molecular, behavioral, electrophysiological, and circuitry studies on SAPAP proteins.
  • To clarify the roles of SAPAP proteins in synaptic function.
  • To provide insights into the mechanistic links between SAPAP dysfunction and neuropsychiatric disorders.

Main Methods:

  • Review of genetic and epigenetic studies.
  • Analysis of in vitro and in vivo animal model data.
  • Integration of molecular, behavioral, electrophysiological, and circuitry findings.

Main Results:

  • Mutations in SAPAP1-4 genes are associated with neurological and psychiatric disorders.
  • SAPAP scaffolding protein dysfunction contributes to the pathogenesis of disorders like schizophrenia, autism, Alzheimer's disease, and bipolar disorder.
  • Recent studies have elucidated SAPAP roles at synapses and their connection to neurodevelopmental and neuropsychiatric conditions.

Conclusions:

  • SAPAP proteins are critical for normal synaptic function and brain activity.
  • Dysregulation of SAPAP proteins is implicated in the pathophysiology of numerous neuropsychiatric disorders.
  • Further research into SAPAPs offers potential therapeutic targets for neurological and psychiatric conditions.