The Citrus Laccase Gene CsLAC18 Contributes to Cold Tolerance.
Xiaoyong Xu1, Yueliang Zhang1, Mengge Liang1
1School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
International Journal of Molecular Sciences
|December 11, 2022
Summary
Plant laccase 18 (CsLAC18) enhances cold stress resistance in plants. This study reveals CsLAC18
Area of Science:
- Plant Science
- Molecular Biology
- Biochemistry
Background:
- Plant laccases are multicopper oxidases involved in plant stress responses.
- The specific role of laccases in plant cold stress tolerance remains largely unexplored.
Purpose of the Study:
- To investigate the function of plant laccases, specifically CsLAC18 from Citrus sinensis, in response to cold stress.
- To elucidate the molecular mechanisms underlying CsLAC18-mediated cold tolerance.
Main Methods:
- Analysis of laccase activity and lignin content in Citrus sinensis under cold treatment.
- Gene expression profiling of Citrus sinensis laccase genes (CsLACs).
- Functional characterization of CsLAC18 through overexpression in transgenic tobacco and gene silencing in Poncirus trifoliata.
Main Results:
- Cold treatment increased laccase activity and lignin content in C. sinensis leaves.
- Cold stress induced differential expression of 21 CsLAC genes, with CsLAC18 showing a significant 130.17-fold increase.
- Overexpression of CsLAC18 enhanced cold tolerance in tobacco, while its suppression impaired cold resistance in Poncirus trifoliata.
Conclusions:
- CsLAC18 positively regulates plant response to cold stress.
- CsLAC18 represents a potential target for developing cold-resilient crops through molecular breeding or gene editing.
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