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Transcriptome Analysis Reveals Immunomodulatory Effect of Spore-Displayed p75 on Human Intestinal Epithelial Caco-2
Soo-Ji Kang1, Ji-Su Jun1, Kwang-Won Hong1
1Department of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University, Goyang-si 10326, Republic of Korea.
International Journal of Molecular Sciences
|December 11, 2022
Summary
Spore-displayed p75 protein from Lacticaseibacillus rhamnosus GG modulates immune responses by activating NF-κB and chemokine pathways in Caco-2 cells. This study reveals key genes and pathways involved in the immunomodulatory action of CotG-p75.
Area of Science:
- Microbiology and Immunology
- Molecular Biology
- Bioengineering
Background:
- Lacticaseibacillus rhamnosus GG (LGG) influences gut immunity, but its soluble factors' immunomodulatory effects are understudied.
- Previous work displayed LGG-derived p75 protein on Bacillus subtilis spores.
Purpose of the Study:
- To investigate the immunomodulatory effects of spore-displayed p75 protein (CotG-p75).
- To analyze the transcriptional response of Caco-2 cells stimulated by CotG-p75 using RNA-sequencing.
Main Methods:
- RNA-sequencing (RNA-seq) to profile gene expression in Caco-2 cells.
- KEGG pathway analysis to identify activated signaling cascades.
- Quantification of cytokine and chemokine expression levels.
Main Results:
- CotG-p75 significantly stimulated genes related to immune regulation, response to stimulus, and chemotaxis.
- Activation of NF-κB signaling and chemokine signaling pathways was observed.
- Increased expression of key immune mediators including CXCL1, CXCL8, CCL20, and IL1B.
Conclusions:
- CotG-p75 influences immune responses through NF-κB and chemokine pathways.
- The study identifies specific genes and pathways modulated by CotG-p75.
- Transcriptome profiling provides insights into the immunomodulatory mechanisms of CotG-p75.
