Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

10.6K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
10.6K
Labeling DNA Probes03:31

Labeling DNA Probes

8.3K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PSA-Responsive Aptamer-Based Switchable Aggregates of Ultrasmall Gold Nanoparticles.

Sensors (Basel, Switzerland)·2026
Same author

Microfluidic Assembly of Poly(glutamic acid) Nanogels Through SPAAC Click Chemistry.

Pharmaceutics·2025
Same author

Spatiotemporal Analysis of Glucagon Secretory Granule Dynamics.

Traffic (Copenhagen, Denmark)·2025
Same author

Fast assembly of 'clickable nanogels' for drug delivery.

Nanoscale advances·2025
Same author

Trajectory Analysis in Single-Particle Tracking: From Mean Squared Displacement to Machine Learning Approaches.

International journal of molecular sciences·2024
Same author

Nanogels: Recent Advances in Synthesis and Biomedical Applications.

Nanomaterials (Basel, Switzerland)·2024
Same journal

RETRACTED: Kim et al. The Angiogenesis Inhibitor ALS-L1023 from Lemon-Balm Leaves Attenuates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease Through Regulating the Visceral Adipose-Tissue Function. <i>Int. J. Mol. Sci.</i> 2017, <i>18</i>, 846.

International journal of molecular sciences·2026
Same journal

Correction: Mahmud et al. Thymoquinone Attenuates NF-κβ Signalling Activation in Retinal Pigment Epithelium Cells Under AMD-Mimicking Conditions. <i>Int. J. Mol. Sci.</i> 2025, <i>26</i>, 11473.

International journal of molecular sciences·2026
Same journal

Correction: Borovikov et al. The Twisting and Untwisting of Actin and Tropomyosin Filaments Are Involved in the Molecular Mechanisms of Muscle Contraction, and Their Disruption Can Result in Muscle Disorders. <i>Int. J. Mol. Sci</i>. 2025, <i>26</i>, 6705.

International journal of molecular sciences·2026
Same journal

Correction: Molagoda et al. Flavonoid Glycosides from <i>Ziziphus jujuba</i> var. <i>inermis</i> (Bunge) Rehder Seeds Inhibit α-Melanocyte-Stimulating Hormone-Mediated Melanogenesis. <i>Int. J. Mol. Sci.</i> 2021, <i>22</i>, 7701.

International journal of molecular sciences·2026
Same journal

Correction: Guo et al. Integrated Transcriptomic and Metabolomic Analysis Reveals the Molecular Regulatory Mechanism of Flavonoid Biosynthesis in Maize Roots Under Lead Stress. <i>Int. J. Mol. Sci.</i> 2024, <i>25</i>, 6050.

International journal of molecular sciences·2026
Same journal

Correction: Chang et al. Improvement of Carbon Tetrachloride-Induced Acute Hepatic Failure by Transplantation of Induced Pluripotent Stem Cells Without Reprogramming Factor c-Myc. <i>Int. J. Mol. Sci.</i> 2012, <i>13</i>, 3598-3617.

International journal of molecular sciences·2026
See all related articles

Related Experiment Video

Updated: Aug 18, 2025

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

9.9K

Choosing the Probe for Single-Molecule Fluorescence Microscopy.

Chiara Schirripa Spagnolo1, Stefano Luin1,2

  • 1National Enterprise for Nanoscience and Nanotechnology (NEST) Laboratory, Scuola Normale Superiore, Piazza San Silvestro 12, I-56127 Pisa, Italy.

International Journal of Molecular Sciences
|December 11, 2022
PubMed
Summary
This summary is machine-generated.

Choosing the right fluorescent probes is crucial for single-molecule microscopy. Careful selection ensures subtle phenomena are not obscured, maximizing the potential of advanced imaging techniques.

Keywords:
fluorescent labelingfluorescent microscopyfluorescent probefluorophoressingle-molecule imaging

More Related Videos

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
08:26

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

2.6K
Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules
10:23

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules

Published on: April 25, 2025

427

Related Experiment Videos

Last Updated: Aug 18, 2025

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

9.9K
Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
08:26

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

2.6K
Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules
10:23

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules

Published on: April 25, 2025

427

Area of Science:

  • Biophysics
  • Optical Microscopy
  • Molecular Imaging

Background:

  • Single-molecule microscopy demands rigorous fluorophore evaluation.
  • Fluorophore properties like brightness, photostability, and spectral characteristics significantly impact data quality.
  • Excitation sources influence sample autofluorescence, complicating single-molecule detection.

Purpose of the Study:

  • To review critical factors in fluorophore selection for single-molecule microscopy.
  • To highlight the impact of fluorophore choice on observing subtle molecular phenomena.
  • To guide effective fluorophore selection for advanced single- and multi-color applications.

Main Methods:

  • Literature review of fluorophore properties and their effects in single-molecule imaging.
  • Analysis of spectral interdependencies between fluorophores and excitation sources.
  • Discussion of fluorophore-related artifacts and their mitigation.

Main Results:

  • Fluorophore characteristics (e.g., nonspecific interactions, brightness, photobleaching, blinking, spectra) critically affect single-molecule observations.
  • Excitation source selection directly impacts observed autofluorescence from the sample.
  • Optimized fluorophore choice is essential for maximizing sensitivity and resolution in single-molecule experiments.

Conclusions:

  • Effective fluorophore selection is paramount for successful single-molecule microscopy.
  • Understanding fluorophore photophysics and spectral properties enhances experimental outcomes.
  • Informed probe choice enables more decisive and demanding single- and multi-color single-molecule experiments.