Comparative Transcriptome Analysis Unveils the Molecular Mechanism Underlying Sepal Colour Changes under Acidic pH
Razieh Rahmati1, Rasmieh Hamid2, Zahra Ghorbanzadeh1
1Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj 3135933151, Iran.
International Journal of Molecular Sciences
|December 11, 2022
Summary
Hydrangea flower color changes are pH-dependent, driven by aluminum accumulation. This study reveals key genes and molecular pathways involved in hydrangea pigmentation, offering insights into flower color regulation.
Area of Science:
- Plant Biology
- Molecular Genetics
- Biochemistry
Background:
- Hydrangea macrophylla exhibits pH-dependent flower color changes, a commercially valuable trait.
- The underlying molecular mechanisms for this phenomenon remain largely unelucidated.
Purpose of the Study:
- To investigate the molecular basis of pH-induced flower color variation in hydrangeas.
- To identify key genes and regulatory networks involved in anthocyanin biosynthesis and aluminum accumulation.
Main Methods:
- Transcriptome sequencing (RNA-seq) and de novo assembly of unigenes.
- Targeted metabolic profiling.
- Weighted gene co-expression network analysis (WGCNA).
Main Results:
- Transcriptomic data revealed dynamic gene expression networks associated with flower color.
- Key genes in anthocyanin biosynthesis (e.g., F3'5'H, ANS, FLS, CHS, UA3GT, CHI, DFR, F3H) were significantly upregulated.
- Hub genes including MYB, bHLH, PAL6, PAL9, and WD40 were identified in modules linked to aluminum hyperaccumulation.
Conclusions:
- A molecular hypothesis for aluminum-induced flower color changes in hydrangeas was proposed.
- This study provides significant insights into the genetic regulation of hydrangea flower pigmentation.
- Identified key regulators offer potential targets for breeding and horticultural applications.
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