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Grapevine Leafroll-Associated Virus 3 Replication in Grapevine Hosts Changes through the Dormancy Stage.

Mate Čarija¹, Silvija Černi², Darija Stupin-Polančec³

¹Institute for Adriatic Crops, 21000 Split, Croatia.

Plants (Basel, Switzerland)

|December 11, 2022

Summary

Grapevine leafroll-associated virus 3 (GLRaV-3) replication is significantly influenced by other grapevine viruses and the plant's phenological stage. Replication is highest during deep dormancy, impacting grapevine production.

Keywords:

grapevine leafroll disease symptoms grapevine viruses qPCR relative quantification virus replication

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Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Published on: December 22, 2017

Area of Science:

Plant Virology
Grapevine Pathology
Molecular Plant Science

Background:

Grapevine leafroll-associated virus 3 (GLRaV-3) is a globally prevalent, graft-transmissible virus threatening grapevine production.
Limited understanding of GLRaV-3 replication dynamics and its interactions with other viruses and host factors hinders effective management.

Purpose of the Study:

To investigate the impact of phenological stage and co-infecting viruses on GLRaV-3 replication in grapevines.
To elucidate viral interactions and their influence on GLRaV-3 symptom expression and replication.

Main Methods:

Graft inoculation of grapevine plants (four varieties) with five different viral inocula (GLRaV-3 alone or with other grapevine viruses).
Quantitative PCR (qPCR) for relative concentration analysis of GLRaV-3 over a 16-month period.
Monitoring of plant phenological stages and symptom expression.

Main Results:

Significant influence of co-infecting viruses and plant phenological stage on GLRaV-3 replication.
GLRaV-3 replication was most pronounced during the deep dormancy stage.
Replication was least significant at the exit of the dormancy stage.

Conclusions:

Plant phenological stage and viral composition are critical factors modulating GLRaV-3 replication in grapevines.
This study provides novel insights into GLRaV-3 replication mechanisms and viral interactions in a key perennial crop.