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Assessment of Intestinal Ischemia-Reperfusion Injury Using Diffuse Reflectance VIS-NIR Spectroscopy and Histology.

Jie Hou1,2, Siri Schøne Ness3, Jon Tschudi4

  • 1Department of Physics, University of Oslo, Sem Sælands vei 24, 0371 Oslo, Norway.

Sensors (Basel, Switzerland)
|December 11, 2022
PubMed
Summary

Visible-near infrared (VIS-NIR) spectroscopy effectively differentiates intestinal ischemia-reperfusion injury in pigs. This non-invasive method accurately assesses viable versus non-viable tissue, aiding intraoperative decisions.

Keywords:
VIS-NIR spectroscopyhistopathologyischemia–reperfusion injurysmall intestine

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Area of Science:

  • Gastroenterology
  • Surgical Innovation
  • Biomedical Optics

Background:

  • Intestinal ischemia-reperfusion injury poses significant clinical challenges.
  • Accurate intraoperative assessment of tissue viability is crucial for patient outcomes.
  • Current methods for evaluating intestinal injury can be invasive or time-consuming.

Purpose of the Study:

  • To evaluate the feasibility of VIS-NIR spectroscopy for assessing degrees of intestinal ischemia-reperfusion injury.
  • To develop and validate spectroscopic models for differentiating healthy, ischemic, and reperfused intestinal tissue.
  • To determine the accuracy of VIS-NIR spectroscopy in distinguishing viable from non-viable intestinal segments.

Main Methods:

  • A porcine model was employed with four intestinal segments: control, 8-hour ischemia, 2-hour ischemia/6-hour reperfusion, and 4-hour ischemia/4-hour reperfusion.
  • VIS-NIR spectroscopy was utilized to acquire spectral data from the intestinal segments.
  • Partial least square discriminant analysis (PLS-DA) was used to build two classification models.

Main Results:

  • The first PLS-DA model accurately differentiated control, ischemic, and reperfused segments (99.2% accuracy).
  • The second PLS-DA model successfully discriminated between viable and non-viable intestinal segments (96.0% accuracy).
  • Histopathology confirmed findings, particularly at the border between viable and non-viable tissue.

Conclusions:

  • VIS-NIR spectroscopy, coupled with PLS-DA, is a promising tool for assessing intestinal ischemia-reperfusion injury.
  • The technique offers a non-invasive, potentially real-time, and easy-to-use intraoperative method.
  • This approach can aid surgeons in making critical decisions regarding intestinal viability during surgery.