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Molecular Level Sucrose Quantification: A Critical Review.

Gustavo A Lara-Cruz¹, Andres Jaramillo-Botero^1,2

¹Omicas Alliance, Pontificia Universidad Javeriana, Cali 760031, Colombia.

Sensors (Basel, Switzerland)

|December 11, 2022

Summary

This review analyzes methods for quantifying sucrose in plants, highlighting limitations in current techniques for real-time, in vivo measurements. It discusses developing advanced biosensors and ligands for improved sucrose detection, crucial for plant science and food security.

Keywords:

artificial receptors biosensors low-molar mass analyte molecular recognition neutral analyte sucrose

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Area of Science:

Plant Physiology
Analytical Chemistry
Biochemistry

Background:

Sucrose is a vital plant metabolite, serving as an energy and carbon source.
Traditional sucrose quantification methods are destructive and lack in vivo, real-time capabilities at ultra-low concentrations.
Understanding sucrose's physiological roles is limited by current analytical constraints.

Purpose of the Study:

To critically analyze existing analytical tools, biosensors, and synthetic ligands for sucrose quantification.
To identify challenges such as signal-noise ratio, stability, and selectivity in current methods.
To discuss promising strategies for improving detection limits for in vivo sucrose sensing.

Main Methods:

Review of current analytical chemistry techniques for sucrose quantification.
Analysis of biosensors and synthetic ligands for molecular recognition of sucrose.
Critical evaluation of methods for in vivo, real-time sucrose detection.

Main Results:

Existing methods for sucrose quantification often require sample destruction and lack real-time, in vivo capabilities.
Challenges in signal-noise ratio, stability, and selectivity limit the use of molecular recognition for in vivo sucrose sensing.
Significant improvements are needed in detection limits for sensitive sucrose quantification.

Conclusions:

Developing real-time, in vivo, highly sensitive, and selective sucrose sensing is crucial.
Advanced sensing capabilities will enhance understanding of plant physiology and cellular processes.
Improved sucrose quantification supports plant breeding for crop productivity, sustainability, and food security.