Theoretical investigation on FRET strategy of ratio metric fluorescent probe sensing hydrogen sulfide

The level of hydrogen sulfide (H₂S) in human body is related to many diseases, such as Alzheimer's disease, Down syndrome, etc. Therefore, the detection of H₂S level in biological systems is very important and has attracted great attention from scientific and clinical researchers. Understanding the design and working mechanism of fluorescent probes for H₂S level detection is important for building new highly efficient fluorescent probe. The mechanisms of a recently reported efficient small molecule fluorescent probe based on the Fluorescence Resonance Energy Transfer (FRET) were investigated thoroughly in this work. The theoretical results would provide the insights for designing new efficient and multi-functional fluorescent probe applicable in the biological systems.