Related Experiment Video
Updated: Aug 17, 2025
08:13
Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting
Published on: April 9, 2019
14.2K
Comparative proteomic analysis of children FSGS FFPE tissues
Jiajia Ni1,2, Sha Tian3, Lin Bai3
1Department of Nephrology, Children's Hospital of Fudan University, National Pediatric Medical Center of China, Shanghai, China.
BMC Pediatrics
|December 12, 2022
Summary
This study identified protein changes in children with steroid-resistant nephrotic syndrome (SRNS) and focal segmental glomerulosclerosis (FSGS). These findings may help find new treatments for pediatric FSGS patients.
Area of Science:
- Nephrology
- Proteomics
- Molecular Biology
Background:
- Focal segmental glomerulosclerosis (FSGS) is a leading cause of steroid-resistant nephrotic syndrome (SRNS) in children.
- Understanding the molecular mechanisms of steroid resistance is crucial for developing effective treatments.
Purpose of the Study:
- To identify protein candidates and elucidate the mechanism of steroid resistance in pediatric FSGS.
- To compare protein profiles between steroid-sensitive (SS) and steroid-resistant (SR) pediatric FSGS patients.
Main Methods:
- Proteomic analysis using liquid chromatography tandem mass spectrometry (LC-MS/MS) on formalin-fixed paraffin-embedded (FFPE) renal tissues.
- Bioinformatic analyses including Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment, and protein-protein interaction (PPI) network construction.
- Immunohistochemistry validation of candidate proteins (LAMP1 and ACSL4) in patient samples and mouse models.
Main Results:
- Over 4000 proteins were quantified, with 325 differentially expressed proteins (DEPs) identified between SS and SR groups (fold change ≥2, P<0.05).
- Up-regulated proteins in the SR group were associated with protein transportation, complement activation, and cytolysis.
- Distinct pathway differences, including lysosome and terminal complement pathways, were observed between the groups. LAMP1 and ACSL4 showed differential expression patterns in renal tissues.
Conclusions:
- This study identified potential protein candidates and mechanisms underlying steroid resistance in pediatric FSGS.
- The findings offer insights into therapeutic targets and outcome prediction for children with FSGS.
- Proteomic profiling provides a valuable approach to understanding complex kidney diseases like FSGS.
