Characterization of immune microenvironment infiltration and m6A regulator-mediated RNA methylation modification
Yulong Ouyang1, Yuanqing Tu1, Shuilin Chen1
1Nanchang University, Nanchang, Jiangxi, China.
Background:
Few studies have been reported the potential role of N6-methyladenosine (m6A) modification in osteoarthritis (OA). We investigated the patterns of m6A modification in the immune microenvironment of OA.
Methods:
We evaluated the m6A modification patterns based on 22 m6A regulators in 139 OA samples and systematically associated these modification patterns with immune cell infiltration characteristics. The function of m6A phenotype-related differentially expressed genes (DEGs) was investigated using gene enrichment analysis. An m6A score model was constructed using principal component analysis (PCA), and an OA prediction model was established based on the key m6A regulators. We used real-time PCR analysis to detect the changes of gene expression in the cell model of OA.
Results:
Healthy and OA samples showed significant differences in the expression of m6A regulators. Nine key m6A regulators, two m6A modification patterns, m6A-related genes and two gene clusters were identified. Some m6A regulators had a strong correlation with each other. Gene clusters and m6A clusters have high similarity, and cluster A corresponds to a high m6A score. Immunocytes infiltration differed significantly between the two clusters, with the m6A cluster B and gene cluster B having more types of infiltrating immunocytes than cluster A. The predictive model can also predict the progression of OA through m6A regulators expression. The results of real-time PCR analysis showed that the gene expression in the cell model of OA is similar to that of the m6A cluster B.
Conclusions:
Our study reveals for the first time the potential regulatory mechanism of m6A modification in the immune microenvironment of OA. This study also sheds new light on the pathogenesis of OA.
Insights
N6-methyladenosine (m6A) modification patterns in osteoarthritis (OA) immune microenvironments were investigated. Key regulators and distinct m6A patterns were identified, offering new insights into OA pathogenesis.
Area of Science:
- Epigenetics
- Immunology
- Molecular Biology
Background:
- Osteoarthritis (OA) pathogenesis is not fully understood.
- The role of N6-methyladenosine (m6A) modification in OA remains largely unexplored.
- Investigating m6A modification in the OA immune microenvironment is crucial.
Purpose of the Study:
- To explore m6A modification patterns within the OA immune microenvironment.
- To identify key m6A regulators associated with OA.
- To establish a predictive model for OA progression based on m6A regulators.
Main Methods:
- Analysis of m6A modification patterns using 22 regulators in 139 OA samples.
- Correlation analysis between m6A patterns and immune cell infiltration.
- Gene enrichment analysis of m6A phenotype-related differentially expressed genes (DEGs).
- Construction of an m6A score model and an OA prediction model.
- Real-time PCR validation in an OA cell model.
Main Results:
- Significant differences in m6A regulator expression between healthy and OA samples were observed.
- Nine key m6A regulators, two m6A patterns, and two gene clusters were identified.
- Distinct immune cell infiltration profiles were found between m6A clusters, with cluster B showing higher infiltration.
- The developed predictive model demonstrated efficacy in predicting OA progression.
- Gene expression in the OA cell model mirrored m6A cluster B patterns.
Conclusions:
- This study elucidates the regulatory role of m6A modification in the OA immune microenvironment.
- The findings provide novel insights into the molecular mechanisms underlying OA pathogenesis.
- m6A modification represents a potential therapeutic target for osteoarthritis.
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