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Updated: Aug 17, 2025

Agarose Fluid Gels Formed by Shear Processing During Gelation for Suspended 3D Bioprinting
Published on: May 26, 2023
An extrusion-based 3D food printing approach for generating alginate-pectin particles
Valentine Barbara J Rysenaer1,2, Safoura Ahmadzadeh1, Filip Van Bockstaele2
1Department of Food Science, University of Arkansas, Fayetteville, AR, 72704, USA.
Abstract:
In the present study, alginate-pectin (Al-P) hydrogel particles containing varied total gum concentrations (TGC) at a constant Al:P ratio of 80:20 were formed utilizing an innovative extrusion-based 3D food printing (3DFOODP) approach. The 3DFOODP conditions, namely, TGC (1.8, 2.0, and 2.2 wt%) and nozzle size (0.108, 0.159, and 0.210 mm) were investigated. The 3DFOODP approach was compared with the conventional bead formation method via a peristaltic pump. All Al-P printing inks exhibited a shear-thinning behavior. The increased apparent viscosity, loss and storage moduli were associated with the increase in the TGC. The size of the wet 3D-printed Al-P hydrogel particles ranged between 1.27 and 1.59 mm, which was smaller than that produced using the conventional method (1.44-1.79 mm). Freeze-dried Al-P particles showed a porous structure with reduced crystallinity. No chemical interaction was observed between alginate and pectin. This is the first report on generating Al-P-based beads using a 3DFOODP technique that can create delivery systems with high precision and flexibility.

