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Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies
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Developing methylotrophic microbial platforms for a methanol-based bioindustry.

Hawaibam Birla Singh1, Min-Kyoung Kang1, Moonhyuk Kwon2

  • 1Division of Applied Life Science (BK21 Four), ABC-RLRC, PMBBRC, Gyeongsang National University, Jinju, South Korea.

Frontiers in Bioengineering and Biotechnology
|December 12, 2022
PubMed
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Methanol bioconversion using methylotrophic organisms offers a sustainable route to produce chemicals. This review highlights advances and challenges in harnessing these microbes for bio-production.

Keywords:
bio-based chemical productionmetabolic engineeringmethanolmethylotrophsmicrobial cell factoriessynthetic biology

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Area of Science:

  • Biotechnology
  • Synthetic Biology
  • Chemical Engineering

Background:

  • Methanol is a cost-effective, renewable C1 feedstock for chemical production.
  • Methylotrophic organisms naturally utilize methanol as a carbon and energy source.
  • Methanol production can utilize greenhouse gases like CO2 and methane.

Purpose of the Study:

  • To review recent advancements in methanol-based bio-production of chemicals.
  • To explore the use of native and synthetic methylotrophic organisms as microbial cell factories.
  • To discuss challenges and future prospects in methylotrophic bioconversion.

Main Methods:

  • Literature review of biotechnological and bioengineering interventions.
  • Analysis of native and engineered methylotrophic pathways.
  • Synthesis of information on bulk and value-added chemical production from methanol.

Main Results:

  • Demonstrated success in producing various chemicals from methanol using methylotrophic platforms.
  • Identified key biotechnological strategies for pathway optimization.
  • Highlighted the potential for greenhouse gas sequestration.

Conclusions:

  • Methylotrophic bioconversion is a promising sustainable strategy for chemical manufacturing.
  • Further optimization of methylotrophic platforms is crucial for industrial viability.
  • Integration of synthetic biology tools enhances methanol utilization efficiency.