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Structural Identification of Eicosanoids with Ring Structures Using Differential Mobility Spectrometry-Electron
Takashi Baba1, J Larry Campbell1, J C Yves Le Blanc1
1Sciex, 71 Four Valley Dr., Concord, Ontario L4K 4V8, Canada.
Abstract:
We developed a structural identification method for eicosanoids with various ring structures using mass spectrometry. We discovered that an electron beam with a kinetic energy of 10 eV, which is in the Electron Impact Excitation of Ions from Organics (EIEIO) regime, cleaved the fatty acids enough to distinguish constitutional and cis/trans isomers. In addition to EIEIO, a comparison to authentic standards using differential mobility spectrometry (DMS) can identify diastereomers, which was difficult by EIEIO. The combination of EIEIO and DMS can provide a high-throughput method to identify complete structures of eicosanoids in mixed samples, which is not allowed with conventional analytical methods though eicosanoids are important signaling molecules in biosystems.
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