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Generation of Insulin-Producing Multicellular Organoids
Laura Mar Fonseca1,2,3, Fanny Lebreton1,2,3, Charles-Henri Wassmer4
1Laboratory of Tissue Engineering and Organ Regeneration, Department of Surgery, University of Geneva, Geneva, Switzerland.
Methods in Molecular Biology (Clifton, N.J.)
|December 12, 2022
Summary
This study details methods for creating insulin-secreting organoids, offering a promising alternative to clinical islet transplantation (CIT) for type 1 diabetes. These organoids aim to overcome CIT
Area of Science:
- Regenerative Medicine
- Endocrinology
- Transplantation Biology
Background:
- Clinical islet transplantation (CIT) for type 1 diabetes (T1D) improves glycemic control but faces limitations like donor scarcity, lifelong immunosuppression, and graft failure due to inflammation and ischemia.
- Organoid generation is a promising regenerative medicine approach to address CIT limitations by enabling scalable production and improved graft survival.
Purpose of the Study:
- To detail a stepwise methodology for generating insulin-secreting organoids using two distinct methods.
- To describe in vitro quality assessment tests for these novel beta-cell replacement therapies.
Main Methods:
- Generation of multicellular insulin-secreting organoids using islet cells and human amniotic epithelial cells (hAECs).
- Detailed step-by-step protocols for organoid fabrication.
- In vitro quality control assays for assessing organoid viability and function.
Main Results:
- Successfully generated 3D insulin-secreting organoid structures.
- Demonstrated improved viability and function of these organoids in vitro.
- Previous in vivo studies showed enhanced graft survival and function.
Conclusions:
- The described methodology provides a reproducible approach for generating insulin-secreting organoids.
- These organoids represent a potential advancement over current clinical islet transplantation for T1D treatment.
- Further research can optimize organoid generation for enhanced therapeutic efficacy and reduced immune rejection.
