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In situ Quantification of Pancreatic Beta-cell Mass in Mice
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MRI Methods for Imaging Beta-Cell Function in the Rodent Pancreas.

Pooyan Khalighinejad1,2, Eul Hyun Suh1, A Dean Sherry3,4,5

  • 1Advanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, TX, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 12, 2022
PubMed
Summary

Researchers developed a new method using a zinc-responsive MRI contrast agent to detect free zinc ions released with insulin from pancreatic beta-cells. This allows real-time monitoring of beta-cell function in live rodents.

Keywords:
Functional imagingGlucose-stimulated insulin secretion (GSIS)Glucose-stimulated zinc secretion (GSZS)Imaging β-cell function in vivoMRIRodent modelsZinc-responsive agents

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Area of Science:

  • Biomedical Imaging
  • Endocrinology
  • Cell Biology

Background:

  • Zinc ions (Zn2+) are crucial for insulin storage within pancreatic beta-cell granules.
  • Insulin secretion, triggered by elevated plasma glucose, also releases free Zn2+ ions.
  • Monitoring beta-cell function is vital for understanding and treating metabolic diseases.

Purpose of the Study:

  • To introduce a novel method for real-time detection of Zn2+ release from pancreatic beta-cells.
  • To demonstrate the utility of a zinc-responsive MRI contrast agent for in vivo imaging.
  • To establish a tool for longitudinal monitoring of beta-cell function in rodents.

Main Methods:

  • Utilized a zinc-responsive magnetic resonance (MR) contrast agent.
  • Administered the agent to rodents to detect local increases in free Zn2+ in the pancreas.
  • Acquired MR images to visualize enhanced "hot spots" indicating Zn2+ release.

Main Results:

  • Successfully detected localized increases in free Zn2+ in the pancreas of rodents in real time.
  • MR imaging revealed clearly enhanced hot spots in the tail of the rat pancreas.
  • The method demonstrated the capacity for longitudinal monitoring of beta-cell activity.

Conclusions:

  • The developed MR imaging technique provides a non-invasive method to monitor beta-cell function.
  • This approach allows for real-time assessment of Zn2+ dynamics linked to insulin secretion.
  • The findings offer a promising tool for studying pancreatic beta-cell physiology and pathology.