A New Mediterranean Flour Moth-Derived Chitosan: Characterization and Co-electrospun Hybrid Fabrication

In this study, the chitin of adult Mediterranean flour moth (Ephestia kuheniella) (Cht) was extracted and then converted to chitosan by deacetylation process to achieve the chitosan derived from E. kuheniella (Chs_fm). The new chitosan-based scaffold was produced using the polyvinyl alcohol (PVA) co-electrospinning technique. The degree of deacetylation was obtained using the distillation-titration and Fourier transform infrared spectroscopy. The surface morphology and crystallinity index of Chs_fm were observed using scanning electron microscopy and X-ray diffraction analysis, respectively, and compared with the commercial chitosan (Chs_c). Thermogravimetric analysis was used to estimate two chitosans' water content and thermal stability. The average molecular mass analysis was performed using viscometry. Moreover, the minimum inhibitory concentration and DPPH assay were used to study the antimicrobial activity and antioxidant potential of the Chs_fm, respectively. Accordingly, Chs_fm was smoother with fewer pores and flakes than Chs_c, and its crystallinity index was higher than Chs_c. The water content and thermal stability were lower and similar for Chs_fm compared to Chs_c. The average molecular mass of Chs_fm was ~ 5.8 kDa, making it classified as low molecular weight chitosan. The antimicrobial activity of Chs_fm against a representative Gram-negative bacteria; E. coli resulted to be the same as Chs_c. However, less effective than Chs_c against a representative Gram-positive bacteria is S. aureus. The Chs_fm/PVA ratio scaffold was optimized at 30:70 to fabricate a uniform nanofiber scaffold.