Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

8.3K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.3K
In-situ Hybridization02:31

In-situ Hybridization

9.5K
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
9.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hepatocyte CD146 and soluble CD146 promote hepatic stellate cell activation via the integrin αvβ1-p38 MAPK signaling axis to drive liver fibrosis.

Cellular and molecular life sciences : CMLS·2026
Same author

Spatial transcriptomic analyses highlight distinct erythroid niches in mice and humans.

Nature genetics·2026
Same author

Developing a pan cancer therapy based on DISE-inducing short RNAs.

Molecular therapy. Nucleic acids·2026
Same author

Immunosenescence in Idiopathic Pulmonary Fibrosis.

Journal of inflammation research·2026
Same author

Efficient hydrogenation of furfural to furfuryl alcohol by recoverable copper tailings catalyst.

RSC advances·2026
Same author

YOLO-Ro-KCF: a lightweight gradient-guided real-time multi-object tracking framework for embedded UAV vision systems.

Scientific reports·2026

Related Experiment Video

Updated: Aug 17, 2025

TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
07:02

TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks

Published on: May 17, 2020

24.4K

Improved probe detection sensitivity by flexible tandem biotin labeling.

Wenjie Cai1, Mingqi Lu1, Xiaoyan Cheng1

  • 1Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou 510642, China; College of Plant Protection, Integrative Microbiology Research Center, South China Agricultural University, Guangzhou 510642, China.

Journal of Microbiological Methods
|December 12, 2022
PubMed
Summary

We developed a new DNA probe labeling method using biotin to enhance detection sensitivity. This technique allows flexible control over signal intensity by adjusting the DNA probe

Keywords:
BiotinEMSANon-isotopeProbe labeling

More Related Videos

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions
08:14

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

Published on: July 17, 2019

12.8K
Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
11:40

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

2.6K

Related Experiment Videos

Last Updated: Aug 17, 2025

TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
07:02

TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks

Published on: May 17, 2020

24.4K
In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions
08:14

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

Published on: July 17, 2019

12.8K
Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
11:40

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

2.6K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Biotechnology

Background:

  • DNA probe detection sensitivity is crucial for various biological assays.
  • Current labeling methods may have limitations in signal amplification.

Purpose of the Study:

  • To develop an improved biotin-based tandem labeling strategy for DNA probes.
  • To enhance the detection sensitivity of DNA probes.

Main Methods:

  • A novel tandem labeling approach using DNA polymerase-mediated overhand filling.
  • Biotinylation of the 3' end of DNA probes.
  • Controlled introduction of poly(A) sequences in the 5' overhang.

Main Results:

  • Successfully achieved tandem labeling of DNA probes with biotin molecules.
  • Demonstrated flexible control over biotin signal intensity.
  • Showcased improved detection sensitivity compared to standard methods.

Conclusions:

  • The developed biotin-based tandem labeling approach significantly enhances DNA probe detection sensitivity.
  • This method offers a tunable signal amplification strategy for molecular detection.
  • Potential applications in diagnostics and research requiring high-sensitivity DNA detection.