Improved probe detection sensitivity by flexible tandem biotin labeling
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Updated: Aug 17, 2025

TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
Published on: May 17, 2020
Wenjie Cai1, Mingqi Lu1, Xiaoyan Cheng1
1Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou 510642, China; College of Plant Protection, Integrative Microbiology Research Center, South China Agricultural University, Guangzhou 510642, China.
Abstract:
We develop a biotin-based tandem labeling approach to improve detection sensitivity of DNA probe. Through DNA polymerase-mediated overhand filling, the 3'end of DNA probe was tandemly labeled with biotin molecules. The intensity of biotin signals could be flexibly manipulated by controlling the introduced length of poly(A) in the 5' overhang.
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