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Updated: Aug 17, 2025

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
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TYMSOS-miR-101-3p-NETO2 axis promotes osteosarcoma progression.

Zun Zhang1, Jin Wang2, Xiaoyan Zhang1

  • 1Orthopaedic Dapartment, Inner Mongolia Baogang Hospital (Third Affiliated Hospital of Inner Mongolia Medical University), No.20 of shaoxian Road, Kundulun District, Baotou, 014010, China.

Molecular and Cellular Probes
|December 12, 2022
PubMed
Summary

The TYMSOS-miR-101-3p-NETO2 axis promotes osteosarcoma (OS) progression by increasing NETO2 expression. Targeting this axis may offer new therapeutic strategies for OS patients.

Keywords:
NETO2OsteosarcomaTYMSOSmiR-101-3p

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Osteosarcoma (OS) is a rare bone cancer primarily affecting adolescents.
  • Neuropilin and tolloid-like 2 (NETO2) is implicated in OS progression, but its regulatory mechanisms are unclear.

Purpose of the Study:

  • To elucidate the upstream regulatory mechanism of NETO2 in osteosarcoma cells.
  • To investigate the functional role of NETO2 in OS cell malignancy.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) and Western blot were used to measure RNA and protein levels.
  • Functional assays assessed the impact of NETO2 on OS cell proliferation, migration, invasion, and apoptosis.
  • Bioinformatics and mechanistic experiments identified the upstream regulation of NETO2.

Main Results:

  • NETO2 depletion inhibited OS cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT), while inducing apoptosis.
  • MicroRNA-101-3p (miR-101-3p) directly targets and downregulates NETO2.
  • Long noncoding RNA TYMS opposite strand RNA (TYMSOS) acts as a miR-101-3p sponge, promoting OS malignancy by upregulating NETO2.

Conclusions:

  • The TYMSOS-miR-101-3p-NETO2 axis drives malignant behaviors in OS cells.
  • This axis represents a potential therapeutic target for osteosarcoma treatment.