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Long non-coding RNA HOXC-AS1 exerts its oncogenic effects in esophageal squamous cell carcinoma by interaction with

Zhengwu Yang1, Junhu Wan1, Liwei Ma1

  • 1Department of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University, and the Key Clinical Laboratory of Henan Province, Henan, China.

Journal of Clinical Laboratory Analysis
|December 13, 2022
PubMed
Abstract

Insights

Long non-coding RNA HOXC-AS1 promotes esophageal squamous cell carcinoma (ESCC) progression. It interacts with insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) to stabilize sirtuin 1 (SIRT1) expression, driving ESCC development.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long non-coding RNA HOXC cluster antisense RNA 1 (HOXC-AS1) is implicated in gastric cancer and nasopharyngeal carcinoma.
  • The role of HOXC-AS1 in esophageal squamous cell carcinoma (ESCC) is currently unknown.
  • RNA-binding proteins (RBPs) like insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) and sirtuin 1 (SIRT1) are involved in cancer progression.

Purpose of the Study:

  • To investigate the function of HOXC-AS1 in ESCC.
  • To elucidate the molecular mechanism of HOXC-AS1 in ESCC, focusing on its interaction with IGF2BP2 and SIRT1.
  • To determine the oncogenic role of the HOXC-AS1-IGF2BP2-SIRT1 axis in ESCC.

Main Methods:

  • In vivo models, HE staining, and immunohistochemistry were used to assess the oncogenic effects of HOXC-AS1.
  • RNA pulldown and RNA immunoprecipitation (RIP) assays verified interactions between HOXC-AS1, IGF2BP2, and SIRT1.
  • Quantitative PCR (qPCR) and actinomycin D experiments analyzed gene expression and stability, while rescue experiments confirmed the axis's functional impact.

Main Results:

  • HOXC-AS1 was found to be highly expressed in ESCC cells and exhibited oncogenic effects in vivo.
  • A positive correlation was observed between HOXC-AS1 and SIRT1 expression.
  • IGF2BP2 was identified as an RBP downstream of HOXC-AS1, binding to SIRT1 mRNA and stabilizing its expression, thereby promoting ESCC progression.

Conclusions:

  • LncRNA HOXC-AS1 plays a significant oncogenic role in ESCC.
  • HOXC-AS1 promotes ESCC progression by interacting with IGF2BP2 to stabilize SIRT1 expression.
  • The HOXC-AS1-IGF2BP2-SIRT1 axis represents a potential therapeutic target for ESCC.
Keywords:
ESCCHOXC-AS1IGF2BP2RNA binding proteinSIRT1

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