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Effect of antimicrobial peptide conjugated surgical sutures on multiple drug-resistant microorganisms

Günnur Pulat1, Zülal Muganlı1, Utku Kürşat Ercan2

  • 1Tissue Engineering and Regenerative Medicine Laboratory, Department of Biomedical Engineering, 226844İzmir Katip Çelebi University, İzmir, Turkey.

Journal of Biomaterials Applications
|December 13, 2022
PubMed

Insights

Novel antimicrobial peptide-conjugated sutures show promise in combating surgical site infections. These advanced sutures accelerate wound healing and offer a potent alternative to traditional antibiotic treatments, addressing rising drug resistance.

Area of Science:

  • Biomaterials Science
  • Infectious Disease Research
  • Wound Healing

Background:

  • Surgical site infections (SSIs) are a significant cause of patient morbidity, often linked to bacterial colonization on surgical sutures.
  • Current antibiotic-coated sutures face challenges due to increasing antimicrobial resistance in pathogens like *Pseudomonas aeruginosa* and *Staphylococcus aureus*.
  • There is a critical need for innovative antimicrobial sutures that are effective against multidrug-resistant bacteria and exhibit low cytotoxicity.

Purpose of the Study:

  • To investigate the antimicrobial efficacy of poly (glycolic acid-co-caprolactone) (PGCL) sutures conjugated with specific antimicrobial peptides (KRFRIRVRV-NH 2 , RWRWRWRW-NH 2 , and their combination).
  • To evaluate the impact of these antimicrobial peptide-modified sutures on keratinocyte cell lines using in vitro wound scratch assays.
  • To assess the potential of these novel sutures as a medical device to combat SSIs and promote wound healing.

Main Methods:

  • Conjugation of antimicrobial peptides (KRFRIRVRV-NH 2 , RWRWRWRW-NH 2 , 1:1 combination) onto poly (glycolic acid-co-caprolactone) (PGCL) sutures.
  • Testing the antimicrobial activity of the modified sutures against *Pseudomonas aeruginosa* and methicillin-resistant *Staphylococcus aureus* (MRSA).
  • Performing in vitro wound scratch assays with keratinocyte cell lines to assess the effect on cell migration and wound closure.

Main Results:

  • Antimicrobial peptide-conjugated PGCL sutures demonstrated significant antimicrobial activity against both *P. aeruginosa* and MRSA strains.
  • The modified sutures showed no adverse cytotoxic effects on keratinocyte cell lines in vitro.
  • Wound scratch assays indicated that the antimicrobial peptide-modified sutures superiorly accelerated wound healing processes.

Conclusions:

  • Antimicrobial peptide-conjugated PGCL sutures represent a promising novel medical device for preventing and treating surgical site infections.
  • These sutures offer a multi-drug resistant antimicrobial solution with enhanced wound healing capabilities.
  • The study highlights the potential of antimicrobial peptides as a viable strategy to overcome the limitations of conventional antibiotic treatments in surgical settings.
Keywords:
Surgical site infectionsantimicrobial peptidesmultiple drug-resistant pathogenssutureswound healing

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