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Related Concept Videos

Riboswitches01:56

Riboswitches

8.3K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
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Types of RNA01:23

Types of RNA

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Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

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Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
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Translational Regulation01:29

Translational Regulation

74
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
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Related Experiment Video

Updated: Aug 17, 2025

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
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Advances in riboswitch-based biosensor as food samples detection tool.

Xiaoze Dong1, Shuo Qi1, Imran Mahmood Khan1

  • 1State Key Laboratory of Food Science and Technology, International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, China.

Comprehensive Reviews in Food Science and Food Safety
|December 13, 2022
PubMed
Summary

Riboswitch biosensing offers enhanced precision and programmability for food safety detection. This review highlights recent advances in riboswitch biosensors for sustainable, real-time food analysis.

Keywords:
aptamerexpressionregulationreporterriboswitchsensor

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Area of Science:

  • Biotechnology
  • Biosensor Technology
  • Food Safety Analysis

Background:

  • Biosensing is crucial for food safety, with riboswitch biosensors offering superior precision and programmability over traditional aptamer methods.
  • Riboswitches control gene expression, enabling conformational changes for signal output, making them ideal for portable, real-time food sample detection.
  • Key features like sustainability and renewability position riboswitch biosensing for transformative engineering applications in food safety.

Approach:

  • This review details the history, definition, and modular design of riboswitch biosensors, including regulatory modes, reporters, and expression platforms.
  • Recent developments in riboswitch biosensors for detecting specific analytes such as theophylline, malachite green, tetracycline, and others are summarized.
  • Guidance for designing effective riboswitch biosensors is provided, alongside an analysis of current challenges and future prospects.

Key Points:

  • Riboswitch biosensors provide a programmable and precise platform for detecting various food contaminants.
  • The modular design allows for customization in regulatory modes, reporters, and expression systems.
  • Numerous applications demonstrate the versatility of riboswitch biosensors across different target molecules.

Conclusions:

  • Riboswitch biosensing represents a significant advancement in food safety detection technology.
  • Further research into challenges and prospects will drive innovation and broader adoption.
  • The review offers a strategic outlook for future developments in this field.