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DACH1 inhibits the proliferation and migration of papillary thyroid carcinoma

Shengru Liang1,2, Qian Xu1, Boyun Liu3

  • 1Department of Endocrinology, Xijing Hospital, Air Force Medical University, Xi'an, China.

Cell Biology International
|December 13, 2022
PubMed

Insights

DACH1, a gene regulating cell growth, is downregulated in papillary thyroid carcinoma and inhibits tumor cell proliferation and migration. Its expression correlates with immune cell infiltration, suggesting a role in modulating tumor immunity.

Area of Science:

  • Molecular Biology
  • Oncology
  • Immunology

Background:

  • DACH1 is part of the retinal determinate gene network (RDGN), regulating gene expression.
  • DACH1 typically inhibits tumor growth, but its function in papillary thyroid carcinoma (PTC) is not well understood.

Purpose of the Study:

  • To investigate the expression of DACH1 in thyroid tissues and its correlation with immune infiltration.
  • To determine the functional role of DACH1 in papillary thyroid carcinoma cell proliferation and migration.
  • To explore DACH1's effect on chemokine expression and tumor immunity.

Main Methods:

  • Utilized The Cancer Genome Atlas (TCGA) and Tumor Immune Estimation Resource (TIMER2.0) databases for expression and immune infiltration analysis.
  • Employed cell viability, colony formation, wound healing, transwell migration, and flow cytometry assays to assess DACH1's impact on cell behavior.
  • Measured CXCL8, CXCL10, and CXCL12 expression using ELISA and real-time PCR.

Main Results:

  • DACH1 expression was lower in thyroid cancer tissues compared to normal tissues and positively correlated with CD8+ T cell infiltration.
  • Overexpression of DACH1 suppressed proliferation and migration in TPC-1 and Bcpap cells, while knockdown enhanced these processes.
  • DACH1 was found to regulate the expression of chemokines CXCL8, CXCL10, and CXCL12.

Conclusions:

  • DACH1 plays an inhibitory role in papillary thyroid carcinoma progression.
  • DACH1 influences tumor immunity by modulating chemokine expression and immune cell infiltration.
  • DACH1 represents a potential therapeutic target for papillary thyroid carcinoma.
Keywords:
DACH1chemokinesmigrationpapillary thyroid carcinomaproliferation

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