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Updated: Aug 17, 2025
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Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast
Published on: January 23, 2012
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Live Cell Imaging of Yeast Golgi Dynamics
Giancarlo Costaguta1, Gregory S Payne2, Lydia Daboussi3
1Department of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|December 13, 2022
Summary
This study presents a novel fluorescence imaging method to observe clathrin-coated vesicle dynamics in live yeast cells. The technique allows real-time visualization of cellular processes at the trans-Golgi network.
Area of Science:
- Cell Biology
- Microscopy Techniques
- Molecular Imaging
Background:
- Live cell imaging enables real-time observation of dynamic cellular processes.
- Understanding vesicle trafficking is crucial for cellular function.
- Clathrin-coated vesicles play key roles in transport at the trans-Golgi network.
Purpose of the Study:
- To develop and describe a fluorescence imaging strategy for live cell visualization.
- To specifically image clathrin-coated vesicle dynamics.
- To observe these dynamics at the trans-Golgi network in Saccharomyces cerevisiae.
Main Methods:
- Utilized fluorescence imaging in live yeast cells (Saccharomyces cerevisiae).
- Focused on a single focal plane for detailed observation.
- Targeted the trans-Golgi network to study vesicle dynamics.
Main Results:
- Successfully imaged clathrin-coated vesicle dynamics in real time.
- Demonstrated the method's efficacy at the trans-Golgi network.
- Established a robust technique for observing intracellular transport.
Conclusions:
- The described fluorescence imaging strategy is effective for studying vesicle dynamics.
- This method can be adapted for diverse live cell imaging applications.
- Provides a valuable tool for real-time analysis of cellular processes.
