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Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
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Generation of GM130 Conditional Knockout Mouse.

Mei Mei1, Shilai Bao2,3

  • 1State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

Methods in Molecular Biology (Clifton, N.J.)
|December 13, 2022
PubMed
Summary
This summary is machine-generated.

Golgi apparatus protein GM130 is crucial for cell function. A new GM130 knockout mouse model allows researchers to study its role in animal physiology and disease progression.

Keywords:
Blastocyst injectionClone screeningES cell electroporationGM130 mini-targeting vectorGap-repaired plasmidHomologous recombinationPop-out vectorRetrievingStem cell targetingStem cells cultureTargeting vector

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • The Golgi apparatus is a dynamic organelle vital for secretory trafficking and cargo modification in eukaryotic cells.
  • Golgi apparatus structural changes and functional disorders are implicated in numerous human diseases.
  • The precise role of GM130 in Golgi stack formation and vesicular transport requires in vivo investigation.

Purpose of the Study:

  • To establish a GM130 conditional knockout mouse model for in vivo studies.
  • To investigate the physiological functions of GM130 and the Golgi apparatus at cellular and animal levels.

Main Methods:

  • Development of a GM130 conditional knockout mouse model using homologous recombination, gene targeting, stem cell screening, and blastocyst injection.

Main Results:

  • Successfully established a GM130 conditional knockout mouse model.
  • The model is suitable for studying GM130 and Golgi apparatus functions in vivo.

Conclusions:

  • The GM130 conditional knockout mouse model provides a valuable tool for understanding the role of GM130 in cellular and animal physiology.
  • Further research using this model can elucidate the involvement of Golgi apparatus dysfunction in human disease progression.