Ligand-Mediated Exfoliation and Antibacterial Activity of 2H Transition-Metal Dichalcogenides

Transition-metal dichalcogenides (TMDs) exists mainly in two polymorphs, namely, 1T (metallic) and 2H (semiconducting). To tailor the characteristics and practical utility of TMDs for different applications, functionalization is essential. In our earlier studies, we have shown that functionalized 1T and 2H MoS₂ exhibit exceptionally high antibacterial activity. The functionalization and related biological applications of other 1T (chemically exfoliated) TMDs were reported, but regarding other 2H TMDs, the functionalization and antibacterial activity are not explored yet. Hence, here we prepared functionalized 2H TMDs such as WS₂, WSe₂, and MoSe₂ other than MoS₂ by using a positively charged thiolate surfactant ligand. Further, functionalized 2H TMDs were utilized for antibacterial activity against Gram-positive and Gram-negative bacteria for a comparative antibacterial analysis. Interestingly, we found disparity in activity among the functionalized 2H TMDs, that is, MoS₂ shows higher activity than WS₂ followed by MoSe₂ and WSe₂. The intracellular reactive oxygen species measurement was found to be in the order MoS₂ > WS₂ > MoSe₂ > WSe₂, which is solely responsible for variation in the activity of functionalized TMDs. These results indicate that the easy functionalization of all types TMDs by using thiol ligand and importance of core material should be considered while designing functionalized material for specific applications.