Protocol for host volatile collection and mosquito behavior assays

The volatile collection step is important for insect behavior assays and in-depth component analysis. Here, we describe how to collect volatiles from animals, humans, and skin microbiota. Furthermore, we detail steps to improve behavioral devices adapted to the different collection techniques. This protocol provides a high-throughput and widely applicable approach to study the attracting or repelling effect of volatiles on mosquitoes. The volatile collection step is easily adaptable and time efficient. For complete details on the use and execution of this protocol, please refer to Zhang et al.¹.