Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

T Cell Activation and Clonal Selection

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A phase 1 trial of HPV16 E7 T-cell receptor-engineered T cells in patients with relapsed/refractory HPV16-positive cancers (KITE-439 trial).

Frontiers in oncology·2026

Same author

HLA micropolymorphisms confine neoantigen conformational adaptability and guide T cell receptor selectivity.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Cancer type-specific variation in patterns of driver alterations across 50,000 tumors.

Cancer cell·2026

Same author

Mitochondrial ATP production promotes T cell differentiation and function by regulating chromatin accessibility.

bioRxiv : the preprint server for biology·2026

Same author

Cancer type-specific variation in patterns of driver alterations across 50,000 tumors.

Cancer cell·2026

Same author

A post-translational regulatory map of chronic antigen-driven human T cell dysfunction.

bioRxiv : the preprint server for biology·2026

Search research articles

Related Experiment Video

Updated: Aug 17, 2025

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Published on: May 9, 2025

Less, but better: A simplified design for T cell redirection and conditional payload delivery.

Korbinian N Kropp¹, Christopher A Klebanoff²

¹Immuno-Oncology Service, Human Oncology and Pathogenesis Program, Memorial Sloan Kettering Cancer Center (MSKCC), New York, NY 10065, USA; Center for Cell Engineering, MSKCC, New York, NY 10065, USA.

|December 13, 2022

Summary

Researchers developed a novel system to engineer T-cells for cancer immunotherapy. This approach redirects T-cell specificity and controls payload delivery, enhancing cancer treatment potential.

More Related Videos

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs

Published on: September 10, 2017

Transduction and Expansion of Primary T Cells in Nine Days with Maintenance of Central Memory Phenotype

Transduction and Expansion of Primary T Cells in Nine Days with Maintenance of Central Memory Phenotype

Published on: March 18, 2020

Related Experiment Videos

Last Updated: Aug 17, 2025

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Published on: May 9, 2025

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs

Published on: September 10, 2017

Transduction and Expansion of Primary T Cells in Nine Days with Maintenance of Central Memory Phenotype

Transduction and Expansion of Primary T Cells in Nine Days with Maintenance of Central Memory Phenotype

Published on: March 18, 2020

Area of Science:

Immunology
Biotechnology
Cancer Research

Background:

Cell-based immunotherapies show promise for cancer treatment.
Engineering immune receptors and accessory molecules is key to advancing these therapies.

Purpose of the Study:

To introduce a modular single vector system for enhanced T-cell-based cancer immunotherapy.
To enable simultaneous redirection of T-cell specificity and activation-gated payload delivery.

Main Methods:

Development of a modular single vector system.
Engineering of T-cells to target cancer antigens.
Implementation of conditional expression of accessory molecules for controlled payload delivery.

Main Results:

The system allows for simultaneous redirection of T-cell specificity towards cancer antigens.
Activation-gated delivery of customizable payloads was achieved.
This modular system offers flexibility for advancing cell-based immunotherapies.

Conclusions:

The introduced modular single vector system represents a significant advancement in cell-based cancer immunotherapies.
This technology holds promise for developing more effective and controlled cancer treatments.