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Antifouling Self-assembled Monolayers on Microelectrodes for Patterning Biomolecules

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Published on: August 25, 2009

Printed Antifouling Electrodes for Biosensing Applications.

Marc Zinggeler¹, Sandra Schär¹, Felix Kurth¹

¹Centre Suisse d'électronique et de Microtechnique SA (CSEM), Tramstrasse 99, Muttenz4132, Switzerland.

ACS Applied Materials & Interfaces

|December 13, 2022

Summary

A novel polymer/carbon nanotube (CNT) composite coating for biosensors offers rapid, scalable fabrication via printing. This antifouling electrode coating maintains performance in complex samples like blood serum, enabling sensitive biomarker detection.

Keywords:

antifouling biofouling biosensor carbon nanotubes (CNTs)point-of-care polymer nanocomposite printed electronics surface fouling

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Published on: November 28, 2017

Area of Science:

Electrochemistry
Materials Science
Biotechnology

Background:

Biosensors using miniaturized electrodes show promise for point-of-care diagnostics.
Electrochemical sensor performance is often limited by surface fouling in complex biological fluids.
Existing composite coatings for antifouling require lengthy and complex fabrication processes, hindering commercialization.

Purpose of the Study:

To develop a novel, rapidly fabricated, antifouling composite coating for electrochemical biosensors.
To demonstrate the efficacy of the coating in preventing protein fouling and maintaining sensor performance.
To enable the sensitive detection of biomarkers in undiluted biological samples.

Main Methods:

A photoreactive antifouling copolymer and carbon nanotubes (CNTs) were combined into an ink for printing onto screen-printed electrodes.
Cyclic voltammetry (CV) and protein fouling experiments were used to characterize the coating's electroactive surface area (EASA) and antifouling properties.
Antibodies against C-reactive protein (CRP) were photochemically immobilized onto the functionalized electrodes for sandwich-immunoassay detection.

Main Results:

The polymer/CNT composite coating exhibited an EASA comparable to uncoated electrodes.
The coating retained over 90% of its initial EASA after 1 hour in concentrated bovine serum albumin solution, while uncoated electrodes lost over 80%.
The fabricated biosensors successfully quantified nanogram-range concentrations of CRP in undiluted human blood serum.

Conclusions:

A fast (<2 h), scalable printing process for antifouling polymer/CNT composite coatings was established.
The developed coating effectively prevents biofouling and maintains electrochemical sensor performance in complex biological matrices.
This platform demonstrates significant potential for developing sensitive and robust electrochemical biosensors for point-of-care applications.