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Circ_0001777 Affects Triple-negative Breast Cancer Progression Through the miR-95-3p/AKAP12 Axis

Zhuorong Chen1, Xumeng Gong1, Chun Cheng2

  • 1Department of Head and neck &Breast Surgery, Yue Bei People's Hospital, Shaoguan City, Guangdong Province, China.

Clinical Breast Cancer
|December 13, 2022
PubMed
Abstract

Insights

Circular RNA_0001777 (circ_0001777) suppresses Triple Negative Breast Cancer (TNBC) by regulating the miR-95-3p/AKAP12 pathway. This finding suggests circ_0001777 as a potential therapeutic target for TNBC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Triple Negative Breast Cancer (TNBC) is an aggressive malignancy with limited therapeutic options.
  • Circular RNA_0001777 (circ_0001777) expression is significantly reduced in TNBC tissues, indicating its potential role as a tumor suppressor.

Purpose of the Study:

  • To elucidate the molecular mechanism of circ_0001777 in TNBC.
  • To investigate the regulatory relationship between circ_0001777, microRNA-95-3p (miR-95-3p), and A-kinase anchor protein 12 (AKAP12) in TNBC cells.

Main Methods:

  • Quantitative real-time fluorescence polymerase chain reaction (qRT-PCR) and Western blot were used to assess gene and protein expression.
  • In vitro cell experiments and in vivo xenotransplantation models were employed to evaluate the functional role of circ_0001777.
  • Bioinformatics and Dual-luciferase reporter assays confirmed the interactions between circ_0001777, miR-95-3p, and AKAP12.

Main Results:

  • Circ_0001777 and AKAP12 expression were downregulated, while miR-95-3p was upregulated in TNBC tissues.
  • Circ_0001777 acted as a sponge for miR-95-3p, and AKAP12 was identified as a direct target of miR-95-3p.
  • Overexpression of circ_0001777 inhibited TNBC cell proliferation, migration, and invasion, an effect partially reversed by miR-95-3p co-transfection and exacerbated by AKAP12 knockdown.

Conclusions:

  • The circ_0001777/miR-95-3p/AKAP12 axis plays a crucial role in regulating TNBC progression.
  • Circ_0001777 functions as a tumor suppressor in TNBC by inhibiting proliferation, migration, and invasion through the miR-95-3p/AKAP12 pathway.
  • Targeting the circ_0001777/miR-95-3p/AKAP12 axis presents a promising therapeutic strategy for TNBC.
Keywords:
AKAP12ProliferationTriple Negative Breast Cancercirc_0001777miR-95-3p

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