Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

EMP3 sustains oncogenic EGFR/CDK2 signaling by restricting receptor degradation in glioblastoma.

Acta neuropathologica communications·2023
Same author

Multiplexed imaging and automated signal quantification in formalin-fixed paraffin-embedded tissues by ChipCytometry.

Cell reports methods·2022
Same author

In-Depth Immune-Oncology Studies of the Tumor Microenvironment in a Humanized Melanoma Mouse Model.

International journal of molecular sciences·2021
Same author

Rare heterozygous GDF6 variants in patients with renal anomalies.

European journal of human genetics : EJHG·2020
Same author

The prognostic role of IDH mutations in homogeneously treated patients with anaplastic astrocytomas and glioblastomas.

Acta neuropathologica communications·2019
Same author

Prebiotic condensation through wet-dry cycling regulated by deliquescence.

Nature communications·2019

Related Experiment Video

Updated: Aug 17, 2025

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research
09:12

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research

Published on: June 10, 2025

347

Highly Multiplexed Immunofluorescence Imaging for Quantitative Spatial Analysis in Tissue Samples with

Thomas D Campbell1, Arne Christians2, Madison A Tyler2

  • 1Canopy Biosciences® - A Bruker Company, Saint Louis, MO, USA. Thomas.Campbell@bruker.com.

Methods in Molecular Biology (Clifton, N.J.)
|December 13, 2022
PubMed
Summary
This summary is machine-generated.

Traditional immunofluorescence assays detect few markers due to spectral overlap. ChipCytometry™ enables detection and quantification of dozens of protein targets with single-cell precision.

Keywords:
ChipCytometry™Multiplexed ImmunofluorescenceSingle-cell proteomicsSpatial multiplexing

More Related Videos

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment
06:05

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Published on: June 2, 2023

8.0K
A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections
07:50

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

Published on: March 30, 2020

8.4K

Related Experiment Videos

Last Updated: Aug 17, 2025

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research
09:12

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research

Published on: June 10, 2025

347
Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment
06:05

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Published on: June 2, 2023

8.0K
A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections
07:50

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

Published on: March 30, 2020

8.4K

Area of Science:

  • Biotechnology
  • Cell Biology
  • Immunology

Background:

  • Traditional immunofluorescence (IF) assays are limited in multiplexing capacity due to spectral overlap.
  • Standard fluorescent imaging lacks the dynamic range for accurate single-cell quantification of protein expression.

Purpose of the Study:

  • To introduce a novel protocol for high-multiplex protein detection and quantification.
  • To overcome limitations of traditional IF assays for single-cell analysis.

Main Methods:

  • Development of an iterative staining protocol.
  • Utilizing ChipCytometry™ for high-resolution imaging.
  • Implementing quantitative single-cell analysis.

Main Results:

  • Demonstration of detection and quantification for dozens of protein targets.
  • Achieved single-cell quantitative precision.
  • Overcame spectral overlap and dynamic range limitations inherent in traditional IF.

Conclusions:

  • ChipCytometry™ offers a powerful solution for high-plex single-cell protein analysis.
  • The iterative staining approach significantly enhances multiplexing capabilities.
  • Enables accurate quantification of protein expression at the single-cell level.