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Differences between HMG1 proteins isolated from normal and tumour cells.

E A Alexandrova1, B G Beltchev

  • 1Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.

Biochimica Et Biophysica Acta
|October 15, 1987
PubMed
Summary

High-mobility-group 1 (HMG1) proteins from tumor cells show distinct modifications like acetylation and poly(ADP)-ribosylation compared to normal liver cells. These changes influence HMG1

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Non-histone chromosomal proteins, including high-mobility-group 1 (HMG1), play crucial roles in DNA structure and gene regulation.
  • Differences in protein properties can arise between normal and cancerous cells, impacting cellular functions.

Purpose of the Study:

  • To compare the biochemical and functional properties of HMG1 proteins isolated from rat liver and Guerin ascites tumor (GAT) cells.
  • To identify specific modifications and structural differences in HMG1 between normal and tumor cells.

Main Methods:

  • Peptide mapping to analyze protein structure.
  • Assays for poly(ADP)-ribosylation and acetylation.
  • In vitro studies using radiolabeled acetate.
  • Analysis of protein oligomerization at physiological ionic strength.

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Main Results:

  • HMG1 from GAT cells displayed unique peptide map differences compared to rat liver HMG1.
  • GAT cell HMG1 was significantly more poly(ADP)-ribosylated and acetylated in vivo and in vitro.
  • HMG1 from GAT cells showed a pronounced ability to form oligomers, unlike the monomeric form from rat liver.
  • Deacetylation abolished the oligomerization ability of GAT cell HMG1.

Conclusions:

  • HMG1 protein properties are significantly altered in Guerin ascites tumor cells compared to rat liver cells.
  • Acetylation and poly(ADP)-ribosylation are key modifications differentiating tumor-associated HMG1.
  • These modifications influence the structural properties, such as oligomerization, of HMG1, potentially impacting its function in tumor cells.